Drug-containing tissue graft as well as preparation method and application thereof

A tissue graft and drug technology, used in tissue regeneration, medical science, prosthesis, etc., can solve the problems of complicated operation and high cost, and achieve the effect of simple process, low cost of raw materials, and good biological activity.

Active Publication Date: 2021-03-16
健诺维(成都)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing method of patch to achieve drug sustained release is to prepare the drug into sustained-release microspheres and then cross-link with the patch, which is complicated and costly.

Method used

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  • Drug-containing tissue graft as well as preparation method and application thereof
  • Drug-containing tissue graft as well as preparation method and application thereof
  • Drug-containing tissue graft as well as preparation method and application thereof

Examples

Experimental program
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preparation example Construction

[0039] This embodiment provides a method for preparing a tissue graft, comprising the following steps:

[0040] (1) Get the umbilical cord and cut it longitudinally, remove the veins and arteries, and prepare a blank tissue graft containing the umbilical cord amniotic membrane and Wharton's jelly;

[0041] (2) introducing the drug solution into the blank tissue graft prepared in step (1) to obtain the drug-containing tissue graft;

[0042] The methods of introduction include injection or immersion.

[0043] Preferably, the injection in the present invention is microneedle injection. Specifically, the microneedle injection includes: using a hollow microneedle array to inject a drug solution into the blank tissue graft.

[0044] Another introduction method of the present invention soaking may include: using a solid microneedle array to insert the blank tissue graft, and then soaking the resulting blank tissue graft in a drug solution.

[0045] In some embodiments, the diamete...

Embodiment 1

[0092] Embodiment 1 prepares tissue grafts of different thicknesses

[0093]1. After obtaining the umbilical cord, store the umbilical cord at -80°C until the donor and the sample are confirmed to be qualified: the qualified donor is a healthy puerpera, which can be known from various inspections before the puerpera gives birth; the sample is collected after the puerpera gives birth. of umbilical cord tissue. Sample qualified means that all processing is carried out in accordance with relevant specifications to ensure that there are no contaminants in the drug-containing tissue graft, that is, to ensure that the collected umbilical cord does not contain HIV-1, HIV-2, HTLV-1, hepatitis B and C , West Nile virus, cytomegalovirus, human transmissible spongiform encephalopathies (eg, Creutzfeldt-Jakob disease), and Treponema pallidum.

[0094] 2. A portion of the umbilical cord is then cut lengthwise (using a scalpel, scissors, or other cutting tool) (the umbilical cord is not cu...

Embodiment 2

[0100] Example 2 Preparation of drug-containing tissue grafts using different embedding methods

[0101] In this example, based on sample 3 prepared in Example 1, different embedding methods were used to prepare drug-containing tissue grafts. The drug used in this example was growth factor bFGF, a drug that promotes tissue healing, and the prepared samples were samples 5. Sample 6, Sample 7.

[0102] The preparation method of sample 5 in this example is as follows: soak sample 3 in the culture solution containing growth factors at a concentration of 5000 IU / mL for more than 3 hours. In order to ensure that the sample fully absorbs the culture solution containing growth factors, the soaking time can be appropriately delayed. The volume ratio composition of the culture solution is: 50% glycerol+50% DMEM.

[0103] The preparation method of sample 6 in this embodiment is as follows: first use the solid microneedle array to process sample 3, the insertion depth is 200 μm, and then...

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Abstract

The invention relates to a drug-containing tissue graft as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) longitudinally cutting an umbilical cord, removing veins and arteries, and preparing a blank tissue graft containing amnion and Wharton jelly; (2) introducing a drug solution into the blank tissue graft prepared in the step (1) to obtain a drug-containing tissue graft; the introducing method comprises injection or soaking. The method disclosed by the invention is simple to operate, and the prepared drug-containing tissue graft has a very good drug slow-release function.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a drug-containing tissue graft and its preparation method and application. Background technique [0002] In the real world, tissue damage is caused by various reasons, and surgical graft materials are often used to repair damaged tissues in clinical practice. The materials constituting the patch include polymer materials and biological materials. Among them, although polymer materials have a certain degree of biocompatibility, as inert materials, due to the lack of biological activity, they will inevitably cause adverse reactions such as rejection, erosion or scarring in the human body. Therefore, the use of products derived from natural sources (eg, humans, non-human primates, pigs or cattle) to repair, reconstruct, replace or supplement damaged tissues has become a hot topic of research and development. [0003] The amniotic membrane isolated from human placenta is a thin ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/36
CPCA61L27/54A61L27/3604A61L27/3691A61L2300/602A61L2430/40A61L2300/404A61L2300/406A61L2300/412A61L2300/42A61L2300/426A61L2300/41
Inventor 林永亮刘艳丽罗锦荣何海娜吴有陵
Owner 健诺维(成都)生物科技有限公司
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