Concentration gradient chip and method for detecting drug sensitivity of bacteria

Abstract

The invention discloses a concentration gradient chip and a method for detecting drug sensitivity of bacteria. A flow resistance gradient chip is innovatively designed, gradient control formed by a conventional gradient chip is simplified, the drug concentration can be automatically generated, and drug sensitivity detection of the bacteria is performed. The chip is provided with a plurality of independent interception chambers, independent micro-droplets can be formed for detecting the drug sensitivity of the bacteria, and the growth conditions of the bacteria under different drug concentration gradients can be conveniently observed in real time, so that the minimum inhibition concentration and drug sensitivity rating of strains are determined. According to the method for detecting the drug sensitivity, a detected strain independently grows under drugs with different concentrations through the chip design of the interception chambers, and cross contamination is effectively avoided. Thedesigned chip also has good expansibility. By adjusting the number of the interception chambers, more dense or sparse concentration gradients can be flexibly controlled to be generated. The chip is easy and convenient to manufacture, control operation and equipment integration are simplified, and cost is saved.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to a method for detecting bacterial drug sensitivity, in particular to a concentration gradient chip and a method for detecting drug sensitivity of bacteria. Background technique [0002] Bacterial drug susceptibility testing based on the traditional flat plate method is a classic drug susceptibility testing method, but it requires a lot of manual operations, and it usually takes 24-48 hours to get the test results [1] . Microfluidic chip technology has the advantages of easy integration, small reagent consumption, and high throughput, and is now widely used in bacterial drug susceptibility testing. Microfluidic chip-based drug susceptibility testing is usually combined with optical or electrical detection principles. This is used to assess the growth status of the bacteria to determine whether the bacteria are resistant [2] . The microfluidic chip can automatically ge...

AI Technical Summary

Problems solved by technology

However, the generation of a large number of uniform micro-droplets requires precise control of the fluid, which requires high coordination and precision between devices.

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