Concentration gradient chip and method for detecting drug sensitivity of bacteria

A concentration gradient and chip technology, which is applied in chemical instruments and methods, biochemical equipment and methods, and laboratory containers, etc., can solve the problems of equipment coordination and high precision requirements, and achieve flexible and compact chip design and cost saving , Design flexible effects

Active Publication Date: 2021-03-16
WESTLAKE UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

However, the generation of a large number of uniform micro-droplets requires precise control of the fluid, which requires high coordination and precision between devices.

Method used

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  • Concentration gradient chip and method for detecting drug sensitivity of bacteria
  • Concentration gradient chip and method for detecting drug sensitivity of bacteria
  • Concentration gradient chip and method for detecting drug sensitivity of bacteria

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Embodiment Construction

[0028] The technical solutions of the present invention will be further explained below in conjunction with the accompanying drawings and specific examples.

[0029] The concentration gradient chip of the present invention is a further innovative design of the flow resistance gradient chip, which simplifies the control of the conventional gradient chip to form a gradient, and can automatically generate drug concentration and perform bacterial drug susceptibility detection. It usually includes a main channel. There are inlets and outlets at the ends of the main channel, and there are liquid storage holes and a number of interception chambers evenly distributed on both sides of the main channel from the front end to the rear end of the main channel. The inlet, outlet, liquid storage hole, and retention chamber The chambers are all communicated with the main channel, and each retention chamber is provided with exhaust holes. Such as figure 1 , 2As shown, the entire chip size of...

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Abstract

The invention discloses a concentration gradient chip and a method for detecting drug sensitivity of bacteria. A flow resistance gradient chip is innovatively designed, gradient control formed by a conventional gradient chip is simplified, the drug concentration can be automatically generated, and drug sensitivity detection of the bacteria is performed. The chip is provided with a plurality of independent interception chambers, independent micro-droplets can be formed for detecting the drug sensitivity of the bacteria, and the growth conditions of the bacteria under different drug concentration gradients can be conveniently observed in real time, so that the minimum inhibition concentration and drug sensitivity rating of strains are determined. According to the method for detecting the drug sensitivity, a detected strain independently grows under drugs with different concentrations through the chip design of the interception chambers, and cross contamination is effectively avoided. Thedesigned chip also has good expansibility. By adjusting the number of the interception chambers, more dense or sparse concentration gradients can be flexibly controlled to be generated. The chip is easy and convenient to manufacture, control operation and equipment integration are simplified, and cost is saved.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to a method for detecting bacterial drug sensitivity, in particular to a concentration gradient chip and a method for detecting drug sensitivity of bacteria. Background technique [0002] Bacterial drug susceptibility testing based on the traditional flat plate method is a classic drug susceptibility testing method, but it requires a lot of manual operations, and it usually takes 24-48 hours to get the test results [1] . Microfluidic chip technology has the advantages of easy integration, small reagent consumption, and high throughput, and is now widely used in bacterial drug susceptibility testing. Microfluidic chip-based drug susceptibility testing is usually combined with optical or electrical detection principles. This is used to assess the growth status of the bacteria to determine whether the bacteria are resistant [2] . The microfluidic chip can automatically ge...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00C12Q1/18B01L3/00
CPCC12M23/16C12M41/36C12M41/46C12Q1/18B01L3/5027B01L2200/10
Inventor 鞠峰周南嘉张慧林张璐姚远惠岳
Owner WESTLAKE UNIV
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