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Beauveria bassiana and application thereof

A technology for Beauveria bassiana and plant diseases, which is applied in the field of agricultural microorganisms, can solve problems such as no report of Beauveria bassiana, and achieves the best control effect and high antibacterial activity.

Active Publication Date: 2021-03-16
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Beauveria bassiana with both antibacterial and siderophore activity has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0026] According to another preferred embodiment of the present invention, the culture is carried out in a steamed rice culture medium, and the culture method is 30-60 days at 25-30° C. under dark conditions. The method may also include extracting the culture product to obtain hydroxamic acid type siderophores and antibacterial active substances therefrom. The method of extraction operation may be methanol leaching, that is, soaking the culture product with methanol. The amount of methanol used may be 0.25-0.5 L per gram of the culture product. The temperature of extraction can be 25-37°C, and the time can be 24-48h.

[0027] In the present invention, adding Fe in the preparation 3+ Afterwards, its antibacterial activity significantly decreased, indicating that there is a close relationship between siderophore activity and antibacterial activity in the bacterial strain of the present invention. Thus, in preferred cases, the formulation is free of iron ions, ie the method do...

Embodiment 1

[0036] This example can illustrate the results of morphological identification and molecular genetic classification identification of the strains involved in the present invention.

[0037] A fungus was isolated from the virgin forest soil (100°49'-101°53'E, 23°95'-24°17'N, altitude 2600m) of Ailaoshan National Nature Reserve, Yunnan Province, China, and its It is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No.21047.

[0038] Cultivate Beauveria bassiana on plate medium (potato 300g, glucose 30g, agar 27g, deionized water 1.5L, pH 7), the colony shape is round, white, opaque, and there are fluffy hyphae on the surface of the colony ; The conidia of the bacterial strain were magnified by 20,000 times with a scanning electron microscope and were sparsely spaced, elliptical, 2 μm-3.5 μm long, and 1.5 μm-2 μm wide, with folds on the surface of the conidia. On the basis of morpho...

Embodiment 2

[0040] This example can illustrate the siderophore-producing performance of the strains involved in the present invention.

[0041] (1) Test tube culture

[0042] Inoculate Beauveria bassiana on a solid slant medium, and culture it at 25°C for 84 hours to obtain a test tube species; the formula of the solid slant medium is: 300 g of potatoes, 30 g of glucose, 27 g of agar, 1.5 g of deionized water L, pH 7;

[0043] (2) Solid plate seed culture

[0044] Cultured on plate culture medium, the method is as follows:

[0045] Preparation of iron-free Chapei solid medium: glucose 30g L -1 , sodium nitrate 2g·L -1 , potassium phosphate trihydrate 1g·L -1 , potassium chloride 0.5g·L -1 , magnesium sulfate heptahydrate 0.5g L -1 , 8-hydroxyquinoline 0.75g L -1 , deionized water 1L, solid plus agar 18g.

[0046] Double-layer chromogenic medium: lower layer: 100ml deionized water, 1.8g agar, 15ml CAS solution; upper layer: iron-free Chapei solid medium. Then inoculate Beauveria ...

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Abstract

The invention relates to the field of agricultural microorganisms, and discloses a beauveria bassiana and an application thereof. The preservation number of the beauveria bassiana disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 21047. In addition, the invention also provides an application of the beauveria bassiana in preparation of a hydroxamic acid type siderophore and regulation of plant diseases. The beauveria bassiana disclosed by the invention is a dominant strain of high-yield siderophore, and can be used for synthesizing and secreting the hydroxamic acid type siderophore. The secondary metabolites of the beauveria bassiana disclosed by the invention have antibacterial activity on pseudomonas solanacearum, pectobacterium carotovorumand ralstonia solanacearum.

Description

technical field [0001] The invention relates to the field of agricultural microorganisms, in particular to a strain of Beauveria bassiana and its application. Background technique [0002] Iron is abundant in soil and the earth's crust, and iron is one of the essential trace elements for almost all organisms in nature. Iron element plays an important role in the enzymatic reaction and metabolic activity of organisms; however, iron element mainly exists in insoluble trivalent stable form in nature, and the content of ferrous iron available to organisms is relatively scarce, and the utilization rate of iron element very low. Iron deficiency is common in plants in agricultural production, which limits the normal growth and development of plants and reduces the resistance of plants. In addition, the imbalance of bacterial abundance in the soil microbial community can aggravate plant diseases. Therefore, in order to adapt to the environment with low content of ferrous ions, mic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02A01N63/30A01P3/00C12R1/645
CPCC12P1/02A01N63/30
Inventor 杨佩文李铭刚邹雪峰赵江源何翔张庆杨济达朱红业施竹凤
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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