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Alcaligenes faecalis DY-8 strain and application thereof in removal of heavy metal cadmium

A technology of Alcaligenes faecalis and DY-8, applied in the field of microbial treatment of heavy metals in soil, can solve problems such as aggravating soil acidification, and achieve the effects of broad application prospects, efficient removal, and simple medium components

Active Publication Date: 2021-03-23
CENT SOUTH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is routine to remove cadmium from soil by lowering soil pH, but this will exacerbate the problem of soil acidification

Method used

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  • Alcaligenes faecalis DY-8 strain and application thereof in removal of heavy metal cadmium
  • Alcaligenes faecalis DY-8 strain and application thereof in removal of heavy metal cadmium
  • Alcaligenes faecalis DY-8 strain and application thereof in removal of heavy metal cadmium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Isolation, purification and identification of bacterial strain (Alcaligenes faecalis DY-8)

[0034] The strain was isolated and purified from a soil sample collected from a heavily cadmium-contaminated paddy field in Xiangtan City, Hunan Province. The details are as follows: add 10 g of fresh cadmium-contaminated soil samples into 100 mL of sterilized deionized water, shake on a vortex shaker for 15 minutes to obtain a microbial suspension, and take 1 mL of the suspension and dilute it to 10 times in sequence with a 10-fold gradient. -4 , 10 -5 , 10 -6 , 10 -7 , 10 -8 times. Weigh 3.6g of nutrient broth (NB) medium into a conical flask, add 4g of agar powder and 200mL of deionized water to maintain the natural pH value, sterilize at 115°C for 30 minutes, pour the plate, and pipette 100 μl of Spread the microbial suspension on the solid medium, culture at 30°C for 24-48 hours, and select a single colony for multiple streak separation. After all the isola...

Embodiment 2

[0039] Embodiment 2: Bacterial strain (Alcaligenes faecalis) DY-8 is to cadmium polluted soil total cadmium and effective cadmium removal effect

[0040] The soil samples were all collected from a heavily cadmium-polluted paddy field in Xiangtan City, Hunan Province: take 0-15 cm of cultivated soil, remove plant residues, mix thoroughly, air-dry at room temperature, and grind through a 100-mesh sieve for later use. The experiment was carried out with a solid-liquid ratio of 1:10 (w / v), and an experimental group (DY-8) and a control group (CK) were set up in the experiment, and the bacterial concentration of the experimental group was 8×10 8 cells / mL of the bacterial solution, the control group was added deionized water with the same pH value (8.45) as the bacterial solution, and leached under the same conditions, with 3 repetitions in each group.

[0041] Weigh 10g to 250mL of the above-mentioned soil samples into conical flasks, add equal volumes of deionized water and bacter...

Embodiment 3

[0043] Embodiment 3: Bacterial strain (Alcaligenes faecalis) DY-8 handles cadmium form change after cadmium polluted soil

[0044] The soil sample after leaching in embodiment 2 is measured soil cadmium form by BCR continuous extraction method, the result is as follows Figure 4 shown. The cadmium in the soil used in the experiment mainly exists in the weak acid state (44.44 mg / kg) and iron-manganese bound state (31.28 mg / kg), and the content of organic bound state (2.31 mg / kg) and residue state (0.534 mg / kg) is relatively high. few. After using bacterial strain (Alcaligenesfaecalis) DY-8 to process five times, the content of four kinds of cadmium forms in the soil of control group and experimental group were respectively: control group 39.91mg / kg (weak acid state), 28.51mg / kg (iron-manganese binding state), 2.15 mg / kg (organic binding state), 0.520mg / kg (residue state), treatment group 14.61mg / kg (weak acid state), 10.22mg / kg (iron-manganese binding state), 0.953mg / kg (orga...

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Abstract

The invention discloses an alcaligenes faecalis DY-8 strain and application thereof in removal of heavy metal cadmium. The preservation number of the alcaligenes faecalis DY-8 strain is CCTCC No:M2020786. The strain is obtained by screening and separating from high-cadmium-contaminated rice field soil; and the pH value of a culture medium reaches 8.25-8.75 in a stable period. When the strain is used for leaching cadmium-contaminated soil, the removal rate of weak acid cadmium, iron-manganese combined cadmium and total cadmium is 65%-75%; the removal rate of organic combined cadmium is 50%-60%;and the pH value of the soil is remarkably increased by 1-2.5 units. The strain realizes treatment of cadmium-contaminated soil under alkaline conditions, relieves the common problem of soil acidification at present while removing the content of heavy metal cadmium, and has great application prospects in the aspects of solving the problems of soil cadmium contamination and acidification.

Description

technical field [0001] The invention belongs to the technical field of microbial control of soil heavy metals, and in particular relates to a strain of Alcaligenes faecalis DY-8 and its application in efficient leaching of soil heavy metal cadmium. Background technique [0002] In recent years, health problems caused by heavy metal cadmium pollution have become increasingly serious, and have become an environmental safety issue of widespread concern worldwide. The farmlands in 25 regions of 11 provinces in my country have been polluted by Cd to varying degrees, and the area of ​​cadmium pollution has reached 13 million hm2 2 . Studies have shown that 1,417 tons of cadmium enters farmland in my country every year. Except for the 178 tons exported through various channels every year, 87% of the cadmium remains in the soil. According to this rate, it is estimated that in 20 years, the soil of all cultivated land in my country will exceed The current standard of 0.3mg / kg. Ther...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C09K17/14C12R1/05
CPCC12N1/20B09C1/10C09K17/14
Inventor 刘学端蒋慧丹邓燕柏连阳刘宏伟江卢华
Owner CENT SOUTH UNIV
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