Method for constructing bronchial asthma model of primate

A technology for bronchial asthma and primates, applied in animal husbandry and other directions, can solve problems such as unfavorable large-scale production and increased model cost, and achieve the effects of stable and controllable model, saving detection cost, and reducing the number of injuries.

Pending Publication Date: 2021-03-26
广东蓝岛生物技术有限公司
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Problems solved by technology

In the report, the modeling time is as long as 282 days, which leads to higher model costs and is not conducive to large-scale production

Method used

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  • Method for constructing bronchial asthma model of primate
  • Method for constructing bronchial asthma model of primate
  • Method for constructing bronchial asthma model of primate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Materials and methods

[0053] 1. Select experimental animals:

[0054] 9 cynomolgus monkeys (5 males, 4 females), aged 2-6 years, weighing 2-5kg, were sourced and bred at Guangdong Landao Biotechnology Co., Ltd. (Experimental animal production license number SCXK (Guangdong) 2019- 0010, license number SYXK (Guangdong) 2018-0191).

[0055] Table 2 Basic information of each experimental monkey

[0056] Numbering monkey number age) weight(kg) gender 1 C150467 3 2.85 male 2 C120492 6 3.75 female 3 C140532 4 3.35 female 4 C160453 2 2.20 male 5 C150505 3 3.00 male 6 C140326 4 3.75 female 7 C150663 3 2.90 male 8 C150013 3 3.35 male 9 C140246 4 4.20 female

[0057] 2. Drug preparation:

[0058] 1) Preparation of house dust mite suspension: House dust mite was purchased from Shanghai Qifa Experimental Reagent Co., Ltd., product number RMB83M-1G.

[0059] Aluminum hydrox...

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Abstract

The invention discloses a method for constructing a bronchial asthma model of a primate. The method comprises the following steps by taking house dust mites as allergens, carrying out first week intraperitoneal injection administration, second week intraperitoneal injection administration, fourth week subcutaneous injection administration, fifth week atomization administration, seventh week intramuscular injection administration, eighth week atomization administration, twelfth week intramuscular injection administration, fifteenth week atomization administration on primates to cause sensitization, and constructing a primate model of bronchial asthma According to the method, the bronchus asthma model of the cynomolgus monkey conforms to the clinical characteristics of asthma in the aspectsof inflammatory cells, serum inflammatory cytokines, airway obstruction characteristics and morbidity symptoms, and the hypersensitivity of a sensitization original skin test is positive, which indicates that house dust mites are sensitized in monkey bodies after modeling.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for constructing a primate animal bronchial asthma model. Background technique [0002] Bronchial asthma is chronic airway inflammation caused by dust mite and other allergens through long-term sensitization. Rodent asthma models in the prior art are relatively mature, but non-human primates are more similar to humans than rodents, and drug research results using non-human primate models can better reflect clinical disease changes and are more It has reference value for clinical drug evaluation. There are few reports on primate experimental animal asthma models in the world, and most of them are made of rodents, and no matter what kind of allergen is used, the induction administration is completed within one month [1-3] . [0003] Most of the existing technologies use ovalbumin (OVA) for sensitization, and the modeling time is too short. Since chicken ovalbumi...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02A01K2227/106A01K2207/20A01K2267/0387
Inventor 叶箭梦李岳锋李广业李欣欣邓志峰邱春梅吴玲环邹朝军韦金涛梁展鹏
Owner 广东蓝岛生物技术有限公司
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