Composite modification of mung bean protein and preparation method of mung bean protein-based simulated egg liquid
A compound modification, mung bean protein technology, used in protein food processing, vegetable protein processing, protein food ingredients, etc., can solve problems such as high viscosity, improve functional properties, good solubility, and avoid excessive protein denaturation.
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Embodiment 1
[0050] Prepare an 8% mung bean protein isolate solution, adjust the pH of the solution to 11 and treat it for 50 minutes. After the pH shift starts, perform ultrasonic treatment, and ultrasonic treatment for 40 minutes under the condition of 20 kHz, then adjust the pH of the solution to 7, then add 0.5% PG enzyme, at 45 ° C Incubate for 20 minutes, finally inactivate the enzyme and freeze-dry to obtain the modified mung bean protein isolate.
Embodiment 2
[0052] Prepare 10% mung bean protein isolate solution, adjust the pH of the solution to 12 and treat for 40 minutes, perform ultrasonic treatment after the pH shift begins, and conduct ultrasonic treatment for 30 minutes under the condition of 20 kHz, then adjust the pH of the solution to 7, then add 0.3% PG enzyme, at 45 Incubate at ℃ for 30 minutes, finally inactivate the enzyme and freeze-dry to obtain the modified mung bean protein isolate.
Embodiment 3
[0054] Prepare an 8% mung bean protein isolate solution, adjust the pH of the solution to 10 and treat it for 60 minutes. After the pH shift begins, perform ultrasonic treatment, and ultrasonically treat it for 40 minutes at 20 kHz, then adjust the pH of the solution to 7, and then add 1% PG enzyme, at 45 Incubate at ℃ for 15 minutes, finally inactivate the enzyme and freeze-dry to obtain the modified mung bean protein isolate.
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