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Anti-tumor drug carrier, preparation method thereof and pharmaceutical preparation containing anti-tumor drug carrier

A technology for anti-tumor drugs and pharmaceutical preparations, applied in the directions of anti-tumor drugs, medical preparations containing active ingredients, drug combinations, etc. Problems such as low yield of micro-particles, to achieve the effect of high uniformity, small particle size and good permeability

Active Publication Date: 2021-03-30
HUBEI SOUNDNY BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microparticles prepared according to the prior art have the defects of low yield and poor particle size uniformity, which limits the large-scale production and in vivo use of microparticles, and also limits the development and application of the pharmaceutical preparation.

Method used

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  • Anti-tumor drug carrier, preparation method thereof and pharmaceutical preparation containing anti-tumor drug carrier
  • Anti-tumor drug carrier, preparation method thereof and pharmaceutical preparation containing anti-tumor drug carrier
  • Anti-tumor drug carrier, preparation method thereof and pharmaceutical preparation containing anti-tumor drug carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Different doses 60 Effect of CO irradiation on the release of tumor cell vesicles

[0037] Experimental materials:

[0038] Lung cancer cells NCI-H292, intestinal cancer cells HCT-116, leukemia cell HL60 three cell lines were purchased from China Typical Savings Center.

[0039] 60 The Co radiation source is a TIL type 60CO treatment machine.

[0040] Nanosight NS300 Granules Tracking Analyzer: Malvin, United Kingdom.

[0041] Flow cytometry: BD FACSCANTO II flow cytometer.

[0042] Raw materials were purchased from Dalian Meilun Biotechnology Co., Ltd., Cisplatin CAS: 15663-27-1; Methadate CAS: 59-05-2; Hydrochloride CAS: 25316-40-9.

[0043] The apoptosis dyeing kit is a Biolegend Annexin V-FITC / PI apoptosis test kit.

[0044] 2. Experimental steps

[0045]Take three tumor cells (lung cancer NCI-H292, intestinal cancer HCT-116, leukemia cell HL60), each cell number is 1 × 10 8 / Dish. The three cells were used for GIBCO RPMI-1640 medium, and 10% of the four s...

Embodiment 2

[0053] Example 2: Comparison of the number and particle diameter of the cell vesicles produced by γ-ray irradiation and UVB irradiation

[0054] Experimental material

[0055] Lung cancer cell NCI-H292, intestinal cancer cell HCT-116, leukemia cell HL60 three cell lines were purchased from China Typical Savings Center CCTCC;

[0056] 60 CO radiation source is a TIL type 60CO treatment machine;

[0057] UVB ultraviolet lamps are Philips UVB 311NM TL 100W / 01 ultraviolet lamp;

[0058] Nanosight NS300 Granules Tracking Analyzer: Malvin, United Kingdom;

[0059] Flow cytometry: BD FACSCANTO II flow cytometry;

[0060] Raw materials were purchased from Dalian Meilun Biotechnology Co., Ltd., Cisplatin CAS: 15663-27-1; Methadate CAS: 59-05-2; Hydrochloride CAS: 25316-40-9.

[0061] 2. Experimental steps

[0062] Gamma ray irradiation group: three cells in Example 1 60 The vesicle production after Co ray irradiation, selects a preferred preparation conditions: 10 Gy irradiation, dose ra...

Embodiment 3

[0067] Example 3 Comparison of pharmaceutical vesicles produced under two preparation conditions on in vitro killing effects of tumor cells

[0068] Experimental material

[0069] Drugs: Raw materials were purchased from Dalian Meilun Biotechnology Co., Ltd., hydrochloride CAS: 25316-40-9.

[0070] Lung cancer cells NCI-H292, intestinal cancer cells HCT-116, leukemia cell HL60 three cell lines were purchased from China Typical Savings Center.

[0071] 60 The Co radiation source is a TIL type 60CO treatment machine.

[0072] UVB ultraviolet lamps for Philips UVB 311NM TL 100W / 01 UV lamp tube

[0073]Nanosight NS300 Granules Tracking Analyzer: Malvin, United Kingdom.

[0074] The apoptosis dyeing kit is a Biolegend Annexin V-FITC / PI apoptosis test kit.

[0075] 2. Experimental steps

[0076] According to the steps of the two preparation methods in Example 2, a polyxin vesicles of three tumor cell sources were prepared by gamma rays and UVB, and the Nanosight NS300 was counted, a...

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Abstract

The invention discloses an antitumor drug carrier and a preparation method thereof. The drug carrier is a cell vesicle derived from apoptotic tumor cells, is produced by irradiating the tumor cells with gamma rays at an intensity of 4-10 Gy for 4-10 minutes to apoptoze and release the cells, and has the advantages of simple preparation, small particle size and high uniformity. The pharmaceutical preparation containing the tumor cell vesicle has better permeability at a tumor site and higher curative effect.

Description

Technical field [0001] The present invention belongs to the field of pharmaceutical preparations, involves an anti-tumor pharmaceutical carrier, a preparation method thereof, and a pharmaceutical preparation containing the pharmaceutical carrier, more particularly to a cell vesicle derived from apoptotic tumor cells as a pharmaceutical carrier, prepared Methods and pharmaceutical preparations containing the cell vesicles. Background technique [0002] Chemotherapy is one of the important means of malignant tumor treatment, especially for medium and late patients. It has always been limited to drugs in the tumor site, and limiting its clinical effect in terms of drugs. [0003] In recent decades, ingenuists and pharmacists, the research and development of new chemotherapy drugs such as high-efficiency and low toxicists have emerged, and many modified chemotherapy drugs and new dosage forms. MicropArticle, MP) is a vesic structure that peels out from the surface of the cell membran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/46A61K33/243A61K31/519A61K31/704A61P35/00
CPCA61K9/5176A61K33/24A61K31/519A61K31/704A61P35/00
Inventor 张一李凯陈彬
Owner HUBEI SOUNDNY BIOLOGICAL TECH