Test strip and method for detecting isocarbophos
A technology of isocarbophos and test strips, which is applied in the field of test strips for detecting isocarbophos, can solve the problems that it is difficult to meet the rapid detection of a large number of samples and on-site samples, the processing is cumbersome and time-consuming, and the detection cost is high, and the detection time is achieved. Short, easy to promote and use, high sensitivity effect
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Embodiment 1
[0040] Embodiment 1 detects the preparation of the test strip of isocarbophos
[0041] The preparation method of this test strip mainly comprises the following steps:
[0042] 1) Prepare a conjugate release pad sprayed with isocarbophos monoclonal antibody-colloidal gold marker;
[0043] 2) preparing a reaction membrane with a detection line coated with isocarbophos hapten-carrier protein conjugate and a quality control line coated with goat anti-mouse anti-antibody;
[0044] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the bottom plate into a test strip.
[0045] The following step-by-step detailed description:
[0046] 1. Synthesis of isocarbophos hapten (synthetic route sees attached figure 1 )
[0047] 1) Take 1.83g of 4-nitrosalicylic acid, add 20mL of isopropanol to dissolve, stir well, add 0.5mL of concentrated sulfuric acid, stir at room temperature for 3h, stop the reac...
Embodiment 2
[0082] The detection of isocarbophos in embodiment 2 fruits and vegetables
[0083] 1. Sample pretreatment
[0084] Take a fresh sample, wipe off the soil, and cut it into pieces smaller than 1cm square; weigh (1.00±0.05)g sample into a 15mL centrifuge tube, add 5mL phosphate buffer, cover it, shake it manually for 30s, and let it stand for 1min. Take the supernatant as the sample solution to be tested.
[0085] 2. Test with test strips
[0086] Use a micropipette to draw 70 μL of the sample solution to be tested and drop it vertically into the sample hole; start timing when the liquid flows, react for 10 minutes, and judge the result.
[0087] 3. Analyze test results
[0088] Negative (-): The color of the T line is darker than that of the C line or consistent with the color of the C line, indicating that the concentration of isocarbophos in the sample is lower than the detection limit, such as image 3 a, 3b.
[0089] Positive (+): T-line color is lighter than C-line or ...
Embodiment 3
[0091] Example 3 sample detection example
[0092] 1. Detection limit test
[0093] Take blank potatoes, Chinese cabbage, carrots, onions, eggplants, peaches, grapes, blueberries, mangoes, and bananas, and add isocarbophos to the final concentrations of 0.025mg / kg, 0.05mg / kg, and 0.10mg / kg, respectively. , take a test strip for testing, and repeat the measurement three times for each sample.
[0094] When using test strips to detect samples of potatoes, Chinese cabbage, carrots, onions, eggplants, peaches, grapes, blueberries, mangoes, and bananas, when the concentration of anhydrous amiphos and its added concentration is 0.025mg / kg, the test strips It shows that the color of T line is darker than that of C line or consistent with the color of C line, and it is negative; when the concentration of isocarbophos is 0.05mg / kg or 0.10mg / kg, the test strip shows T The color of the line is lighter than the color of the C line or the T line is not colored, which is positive, indicat...
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