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Islet organ with immune tolerance function as well as preparation method and application thereof

A technology of immune tolerance and organoids, which is applied in the field of islet organoids and its preparation, and islet transplantation, to improve the effect of transplantation, reduce destruction and inflammatory damage, and improve blood sugar control

Inactive Publication Date: 2021-04-06
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Transplanting the islet organoids to diabetic recipients can solve the host’s immune rejection and early ischemia of islet transplants, prolong the survival of the grafts, and improve the effect of blood sugar control after transplantation

Method used

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  • Islet organ with immune tolerance function as well as preparation method and application thereof
  • Islet organ with immune tolerance function as well as preparation method and application thereof
  • Islet organ with immune tolerance function as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1: materials used are as follows:

[0021] 1. The culture medium containing porcine islet cell mass 5000 / mL and the culture medium containing mesenchymal stem cell MSCs 5×10 5 individual / mL.

[0022] 2. Rapamycin (Sigma) 2mM; CTLA4-Ig fusion protein (Bio-X-Cell, catBP0099): 1mg / mL; HMGB1 neutralizing antibody (Shino-Test): 0.2mg / mL.

[0023] 3. Rat tail collagen I solution 5mg / mL.

[0024] 4. Sterile fresh 7.5% (w / v) sodium bicarbonate solution or 1N NaOH solution.

[0025] The preparation method of the islet organoid with immune tolerance function comprises the following steps (the total volume of the islet organoid with immune tolerance function produced according to this protocol is 2.5 mL):

[0026] 1. Use 0.5 mL each of the culture medium containing islet cell clusters and mesenchymal stem cells to fully mix together, and the volume at this time is 1 mL.

[0027] 2. Add 112.5 uL of CTLA4-Ig fusion protein and HMGB1 neutralizing antibody, and 25 uL of...

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Abstract

The invention discloses an islet organ with an immune tolerance function as well as a preparation method and an application thereof. The preparation method comprises the following steps: mixing islet-containing cell clusters, mesenchymal stem cells, an immunosuppressant and a scaffold material according to a volume ratio of 1: 1: 0.5: 2.5 to prepare a hydrogel material, and carrying out a cross-linking reaction for 1 h; the islet organ prepared by the invention can well solve the problems of immunological rejection and early ischemia of a host, prolong the function and survival time of an islet graft, improve the effect of clinical islet transplantation, and relieve the pain of patients suffering from type I diabetes in need of long-term insulin treatment.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the technology of islet transplantation, and specifically relates to a pancreatic islet organoid with immune tolerance function, its preparation method and application. Background technique [0002] At present, islet transplantation is widely considered to be the most potential and effective treatment for type 1 diabetes, and has become a research hotspot in recent years. Multi-center clinical islet transplantation trials have proved that the short-term effect of islet transplantation is remarkable, and the success rate of weaning off insulin therapy at 1 year after transplantation is as high as 80%, but unfortunately, the success rate of weaning off insulin at 2 years after transplantation is less than 15%. Host immune rejection and early ischemia are the main causes of islet graft failure. In order to solve these two problems, there are existing researches on encapsulating islets ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/24A61L27/38A61L27/22A61L27/54
CPCA61L27/52A61L27/3886A61L27/54A61L27/24A61L27/227A61L2300/216A61L2300/426
Inventor 何斯荣柏雪王彬王丹
Owner CHONGQING MEDICAL UNIVERSITY
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