Paper-based detection method for exosome

A detection method and technology of exosomes, which are used in the determination/inspection of microorganisms, biochemical equipment and methods, etc.
CN112608983AActive Publication Date: 2021-04-06CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2021-04-06

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Abstract

The invention provides a paper-based detection method for exosome. The paper-based detection method employs (1) a recognition and molecular transformation system, (2) an amplification reaction system and (3) an LFB detection and analysis system. According to the invention, the exosome is converted into a single-stranded DNA target; the single-stranded DNA is amplified by a nucleic acid amplification technology; and the amplified single-stranded DNA is further detected by using lateral flow chromatography test paper which uses PtAu-NiO2 nano-enzyme as a signal probe. The nucleic acid lateral flow chromatography test paper is used as a terminal detection platform, so the problem that the exosome cannot be qualitatively and quantitatively detected at the same time is solved, and simple, convenient and low-cost detection of the exosome is realized.
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Description

technical field

[0001] The invention belongs to the technical field of biological detection, and in particular relates to a paper-based detection method for exosomes. Background technique

[0002] The nanoscale particle size and ultra-trace concentration of exosomes in body fluids pose great challenges for their identification and analysis. Existing analysis methods not only rely on large and expensive instruments, but also require complex pretreatment processes and professional operators. In addition, traditional exosome detection methods cannot be qualitative and quantitative at the same time. Therefore, the development of fast, simple, low-cost, and highly sensitive exosome detection methods is the key to the rapid development and popularization of clinical disease diagnosis and treatment methods using exosomes as biomarkers.

[0003] Lateral Flow Chromatography (LFB) is a sensitive, cheap and simple visual rapid detection method. Early LFBs were based on immune recogni...

Claims

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