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Bacillus amyloliquefaciens Z-2 strain for preventing and treating root rot of fruits and vegetables, and application of bacillus amyloliquefaciens Z-2 strain

A technology for dissolving starch spores, Z-2, applied in the field of microorganisms, can solve the problems of polluted environment, high toxicity, high residue and so on

Active Publication Date: 2021-04-20
河北冀微生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the technical problems of high toxicity, high residue, environmental pollution and many other disadvantages of chemical prevention and control of vegetable root diseases, the present invention provides a Bacillus amyloliquefaciens Z-2 strain for the prevention and treatment of fruit and vegetable root rot and its application

Method used

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  • Bacillus amyloliquefaciens Z-2 strain for preventing and treating root rot of fruits and vegetables, and application of bacillus amyloliquefaciens Z-2 strain
  • Bacillus amyloliquefaciens Z-2 strain for preventing and treating root rot of fruits and vegetables, and application of bacillus amyloliquefaciens Z-2 strain
  • Bacillus amyloliquefaciens Z-2 strain for preventing and treating root rot of fruits and vegetables, and application of bacillus amyloliquefaciens Z-2 strain

Examples

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Effect test

Embodiment 1

[0032] The embodiment of the present invention provides a kind of Bacillus amyloliquefaciens Z-2 bacterial strain that prevents and cures fruit and vegetable root rot, and this bacterial strain is obtained by screening through the following steps:

[0033] 1. Media and reagents

[0034] 1.1 Medium formula

[0035] Potato dextrose (PDA) medium: 200 g of potatoes, 20 g of glucose, 15-20 g of agar, and 1000 mL of distilled water. Peel the potatoes, cut them into small pieces, add water to boil for 0.5h, filter with double gauze, take the filtrate, add glucose and agar, and add water to make up 1000mL.

[0036] Nutrient broth agar (NA) medium: beef extract 5g, peptone 10g, NaCl 5g, agar 15-20g, distilled water 1000mL, pH7.0-7.2.

[0037] Nutrient broth (NB) medium: beef extract 5g, peptone 10g, NaCl 5g, distilled water 1000mL, pH7.0~7.2.

[0038] Seed medium: peptone 1%, glucose 1%, NaH 2 PO 4 2H 2 O 0.2%, Na 2 HPO 4 2H 2 O 0.4%, MgSO 4 ·7H 2 O 0.05%, the balance is wat...

Embodiment 2

[0077] This example provides an understanding of the field test effect evaluation of the Bacillus amylobacter Z-2 strain on the control of fruit and vegetable root rot.

[0078] Inoculate the Bacillus amyloliquefaciens Z-2 strain into the NA slant medium, culture at 37°C overnight (16h) for activation, pick the activated bacterial lawn into a 250mL Erlenmeyer flask containing 50mL NB medium, and shake it on a shaker at 37°C Cultivate overnight (16h), inoculate the culture into 100mL NB medium with a 10% inoculum size, culture on a shaker at 37°C for 48h, and detect the spore content in the fermentation broth with a bacterial counting plate. Use sterile water to adjust the concentration of spores in the fermentation broth to 1×10 9 cfu / mL, namely the fermentation broth of Bacillus amyloliquefaciens Z-2 strain.

[0079] The common fruits and vegetables strawberry, cucumber and tomato were selected as the detection objects, and the control effect of Bacillus amyloliquefaciens Z-...

Embodiment 3

[0096] This embodiment provides a bacterial agent, which is the fermented product of Bacillus amyloliquefaciens Z-2 bacterial strain. Slant culture medium, 37°C constant temperature culture overnight (16h) for activation, pick the activated bacterial lawn and inoculate it in NB medium, 37°C constant temperature shaking culture overnight (16h), inoculate the bacterial liquid in NB medium with 10% inoculum , 37 ℃ constant temperature shaking culture for 48h, with sterile water to adjust the concentration of spores in the fermentation broth to 0.5 × 10 9 ~1.5×10 9 cfu / mL, potting, ready to use.

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Abstract

The invention relates to a bacillus amyloliquefaciens Z-2 strain for preventing and treating root rot of fruits and vegetables, and application of the bacillus amyloliquefaciens Z-2 strain. The strain is preserved in the China General Microbiological Culture Collection Centre (CGMCC) on September 25, 2020; and the preservation number is CGMCC No.20822; and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bacillus amyloliquefaciens Z-2 strain has a remarkable antagonistic effect on common pathogenic bacteria causing root rot of fruits and vegetables, and can effectively prevent and treat root rot of fruits and vegetables. Meanwhile, the bacillus amyloliquefaciens Z-2 strain also has relatively strong capability of producing 3-indoleacetic acid and siderophore, and can promote the absorption of plants to nutrient substances, thereby promoting the growth of the plants.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Bacillus amyloliquefaciens Z-2 for preventing and treating fruit and vegetable root rot and an application thereof. Background technique [0002] Root rot is one of the most serious soil-borne diseases in facility fruit and vegetable production, especially root diseases of Cucurbitaceae and Solanaceae vegetables such as cucumbers and tomatoes are particularly serious, generally resulting in a yield loss of more than 10%-15%, sometimes up to 30%-40% or more, or even no income. Root rot mainly occurs at the stem base and roots of plants, and can occur throughout the growth period, invading the root vascular tissues of plants and causing plant death, and root rot caused by Fusarium spp. is particularly common. It has been reported that F.oxysporium is the dominant species causing soil-borne diseases. [0003] At present, the control of vegetable root diseases is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00A01G7/06A01G13/00C12R1/07
Inventor 张冬冬肖嘉文张学超陈晓萌
Owner 河北冀微生物技术有限公司
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