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High-sensitivity helicobacter pylori detection kit

A technology of Helicobacter pylori and detection kit, which is applied in the fields of medical devices and medicine, can solve the problems of poor stability of urea solution, inability to detect Helicobacter pylori quickly, and increase in color change of acid-base indicator, etc.

Inactive Publication Date: 2021-04-20
南京康容健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following disadvantages: (1) Helicobacter pylori produces urease, and it takes a certain amount of time for urease to decompose urea, so that inspectors cannot quickly detect Helicobacter pylori
The application solves the following problems: (1) the culture medium in the substrate solution can allow Helicobacter pylori to obtain nutrition, thereby rapidly producing urease, thereby greatly shortening the detection time; (2) the ammonia absorbent in the substrate solution can make Reaction equilibrium towards NH 4 When the OH end moves, the pH value of the substrate solution changes significantly, and the color change difference of the acid-base indicator increases; (3) the color change range of the general acid-base indicator is relatively large, such as phenol red. 6.6 to 8.0, when the pH is between 6.6 and 8.0, it will show orange, when the pH is lower than 6.6, it will be yellow, and if the pH is higher than 8.0, it will be red, with a difference of 1.4, and there is a gradual change in the intermediate transition color, and urease decomposes urea to make the pH of the substrate solution The value change will be less than 0.5 to a large extent, so when an acid-base indicator like phenol red is used to judge the presence of Helicobacter pylori, the critical point cannot be judged, resulting in misjudgment
It can be seen that the stability of urea solution is poor, which will cause clinical false positive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Dissolve 2 parts of urea, 0.5 part of sodium citrate, 0.02 part of acetic acid / sodium acetate, and 0.2 part of 199 medium in 100 parts of 25% ethanol to prepare solution A, and take 0.5 part of sodium citrate, 199 medium 0.2 parts were dissolved in 100 parts of 25% ethanol to prepare negative solution B, and the pH values ​​of both solution A and negative solution B were adjusted to 3.5. Another part of 0.1% bromocresol green sodium salt solution and one part of 0.2% methyl orange aqueous solution were mixed to adjust the pH to 3.5. Color-correct the indicators with pH values ​​of 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5 and mark them as 0, 1, 2, 3, 4, 5, 6, 7, respectively.

Embodiment 2

[0024] Dissolve 3 parts of urea, 0.3 part of sodium tartrate, 0.03 part of phosphoric acid / sodium phosphate, and 0.25 part of 1640 medium in 100 parts of 30% ethanol to prepare solution A, and take 0.3 part of sodium tartrate and 0.25 part of 1640 medium Dissolve it in 100 parts of 30% ethanol to prepare negative solution B, and adjust the pH values ​​of both solution A and negative solution B to 4.5. Another part of 0.1% chlorophenol red sodium salt solution and one part of 0.1% aniline blue aqueous solution were mixed to adjust the pH to 4.5. Color-correct the indicators with pH values ​​of 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5 and mark them as 0, 1, 2, 3, 4, 5, 6, 7, respectively.

Embodiment 3

[0026] Take 2.5 parts of urea, 0.3 part of nickel chloride and 0.2 part of DMEM medium and dissolve them in 100 parts of 25% ethanol respectively, and prepare solution A, and take 0.3 parts of nickel chloride and 0.2 parts of DMEM medium and dissolve them in 100 parts of 25% ethanol. % ethanol, prepared as negative solution B, the pH values ​​of solution A and negative solution B were adjusted to 6.0. Another part of 0.1% bromocresol purple sodium salt solution and one part of 0.1% bromothymol blue sodium salt solution were mixed to adjust the pH to 6.0. Color-correct the indicators with pH values ​​of 6.0, 6.5, 7.0, 7.5, 8.0, 8.5 and mark them as 0, 1, 2, 3, 4, 5, 6, respectively.

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Abstract

According to a high-sensitivity helicobacter pylori detection kit provided by the invention, the detection time is shortened by adopting a rapid culture method, and the kit also uses an ammonia absorbent to improve the detection accuracy and reduce the misjudgment rate of clinical medical personnel. The kit can be used for large-scale clinical screening to prevent helicobacter pylori infection and propagation.

Description

technical field [0001] The application relates to the field of medical equipment, is a kit for detecting Helicobacter pylori, and belongs to the field of medicine. Background technique [0002] Helicobacter pylori lives in the pyloric part of the human stomach and the oral cavity, and is one of the most common bacterial pathogens. Half of the world's population has been infected with Helicobacter pylori, and in some countries almost 90% of the population has been infected with this bacterium. People are usually infected at an early age, reaching 50% under the age of 5 years. This bacterial infection first causes chronic gastritis, leading to gastric ulcer and gastric atrophy, and in severe cases, it develops into gastric cancer. [0003] According to statistics, the incidence of atrophic gastritis and gastric cancer is higher in people who are first infected with Helicobacter pylori at an earlier age, and there is a parallel relationship between Helicobacter pylori infecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 张广明
Owner 南京康容健康科技有限公司
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