Colorimetric biosensor-based fecal helicobacter pylori rapid detection kit
A technology of Helicobacter pylori and biosensor, applied in the field of Helicobacter pylori detection, can solve problems such as influence, and achieve the effect of low detection cost and good specificity
Active Publication Date: 2022-07-05
SICHUAN UNIV
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However, the detection accuracy of the C13 breath test method is easily affected by some factors, such as diet and drugs
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[0020] The AuNPs used in this example were characterized by UV-Vis spectrophotometer and transmission electron microscope (TEM). see attached figure 1 , where A is the absorption peak of AuNPs solution, B is the size and morphological characteristics of AuNPs; it can be seen that AuNPs has a characteristic absorption peak at 530 nm, and no other impurity peaks appear, which has good specificity and stability; TEM results showed that AuNPs dispersed Good, spherical particles about 20 nm in diameter.
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Abstract
The invention relates to the technical field of helicobacter pylori detection, and discloses a coliform helicobacter pylori rapid detection kit based on a colorimetric biosensor, the kit comprises a helicobacter pylori specific nucleic acid aptamer and oligonucleotide probes modified by gold nanoparticles (AuNPs), the oligonucleotide probes comprise a probe Probe-120 and a probe Probe-220, and the probe Probe-120 and the probe Probe-220 are used for detecting the helicobacter pylori specific nucleic acid aptamer. The 5'end of the Probe-120 and the 3 'end of the Probe-220 are respectively modified with sulfydryl (SH). According to color change of AuNPs in the aggregation and depolymerization process, detection of helicobacter pylori (25CFU / ml) with low concentration is achieved, good specificity is achieved, compared with a C13 expiration detection method, the method has the advantages of being low in detection cost, short in detection time, free of radioactivity and the like, and the method is suitable for popularization and application. Therefore, the method is expected to become a new means for detecting helicobacter pylori infection in clinic.
Description
technical field [0001] The invention relates to the technical field of Helicobacter pylori detection, in particular to a fecal sample Helicobacter pylori rapid detection kit based on a colorimetric biosensor. Background technique [0002] Helicobacter pylori is a gram-negative bacterium, which is closely related to the occurrence of many digestive tract diseases such as chronic gastritis, peptic ulcer and gastric cancer. It is a class I carcinogen announced by the World Health Organization. The global infection rate of Helicobacter pylori is about 41%, while the infection rate of Helicobacter pylori in the Chinese population is as high as 59%. [0003] At present, the detection methods for Helicobacter pylori can be roughly divided into two types: invasive and non-invasive. Non-invasive detection (NID) methods include stool antigen test (SAT), antibody-based test (ABT) and C13-urea breath test. C13-UBT) and so on. Among them, the C13 breath detection method has become the...
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IPC IPC(8): G01N33/569C12Q1/6816C12N15/11
CPCG01N33/56911C12Q1/6816C12Q2525/205C12Q2563/137C12Q2563/155Y02A50/30
Inventor 唐田周琛方楚斌费宇方蓉肖丽娜廖娟汪川
Owner SICHUAN UNIV
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