Paranosema locustae strain and preparation and use methods

A technology for locust microsporidia and strains, which is applied in the field of locust microsporidia strains and preparation, can solve the problems of killing and injuring beneficial animals, poisoning of humans and animals, low temperature resistance, etc. The effect of good pathogenicity and strong resistance to high temperature

Pending Publication Date: 2021-04-27
北京中维新兴技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the main method of controlling locusts is to use chemical pesticides. Although chemical control can quickly reduce the population density of locusts, it often causes adverse consequences such as polluting the environment, killing a large number of beneficial animals, poisoning humans and animals, and developing insecticide resistance.
[0003] Since the 1970s, black locusts were used as breeding hosts abroad to cultivate No. High cost, short storage period, harsh environmental conditions for use, unsafe for some organisms, etc., which limit the scope of use, and the locust microsporidia strains that have been reported at home and abroad are resistant to adverse external environments, especially high temperature resistance relatively low

Method used

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preparation example Construction

[0024] The technical scheme that the present invention adopts is as follows: a kind of locust microsporidia strain preparation method comprises the following steps:

[0025] 1) Quarantine and rejuvenation after locusts are collected in the wild;

[0026] 2) species preservation of the rejuvenated locusts in step 1);

[0027] 3) carry out locust ovum hatching to the species-preserving locust in step 2), and healthy insects are raised;

[0028] 4) Healthy worms are inoculated with the spores of Microsporidia, and the plant blades coated with the spore liquid of the Microsporidia locust PL-GM1 strain are fed to the locust gnats;

[0029] 5) Feeding of diseases and insect pests, use normal plant leaves to feed until death;

[0030] 6) Microspore processing and inspection;

[0031] 7) Product packaging quality inspection and frozen storage.

[0032] The technical scheme that the present invention adopts is as follows: a kind of locust microsporidia strain use method comprises t...

Embodiment 1

[0044] Embodiment 1, the acquisition of Microsporidia locust PL-GM1 strain

[0045] Because of the high temperature in the tropics, the locust microsporidia strains that can be prevalent in tropical locust swarms all have strong high temperature resistance. The sporozoite strain (National Pesticide Standardization Technical Committee named Microsporidia locust PL-GM1, the strain has been preserved with the General Microbiology Center of the China Microbiological Culture Collection Management Committee, registration number 19390) has strong high temperature resistance and is virulent. The disease power and pathogenicity are good, and the effect of controlling a variety of main locusts is stable.

[0046] On April 3, 2020, the PL-GM1 strain of Paranosema locustae was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures (CGMCC for short, address: Yard 3, No. 1 Beichen West Road, Chaoyang District, Beijing No.), the deposit numb...

Embodiment 2

[0047] Embodiment 2, the pathogenicity identification of locust microsporidia PL-GM1 strain

[0048] The Microsporidia locust PL-GM1 strain can be used to control a variety of locusts, such as prairie locusts, migratory locusts, bamboo locusts, and rice locusts. The pathogenicity and pathogenicity are strong, and it can be vertically transmitted to the next generation, which is conducive to the long-term prevalence of locust microsporidiosis in locust swarms, showing a good continuous control effect.

[0049] The locust Microsporidia PL-GM1 strain is effective against the dominant species of locusts in my country: Migratory locust, Asian locust, Italian locust, Northwest Asia locust, broad-bearded ant locust, drum-winged wrinkle-knee locust, yellow-ridged bamboo locust, Chinese rice locust, etc. All have specific lethal effect, inoculate 3×10 6 After 3 weeks of spores, the mortality rates to the above locusts reached 95.3%, 93.6%, 89.7%, 91.2%, 96.7%, 87.3%, 91.2% and 96.1%, r...

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Abstract

The invention discloses a Paranosema locustae strain and preparation and use methods. Paranosema locustae is called after PL-GM1, is separated and screened from Hainan native grasshoppers, has been collected in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms (CGMCC for short, address: 3#, courtyard 1#, west North Star road, Chaoyang district, Beijing) on April, 3rd 2020 and has an accession number of CGMCC No. 19390. The Paranosema locustae PL-GM1 CGMCC No. 19390 is PL-GM1 for short. The invention belongs to the technical field of grasshoppers and particularly relates to the Paranosema locustae strain which has the advantages that the Paranosema locustae strain is screened from native land, has a good effect on the grasshoppers and the like, is high in capability such as high-temperature resistance and storage resistance, the shelf life is prolonged remarkably, and the grasshopper control effect is improved remarkably.

Description

technical field [0001] The invention belongs to the field of locust technology, and specifically refers to a locust microsporidia strain and a preparation and use method. Background technique [0002] The locust plague is a worldwide natural disaster, which has had a great impact on agricultural production. Over the years, there have been frequent outbreaks of locust plagues in large areas, including soil locusts, migratory locusts, rice locusts, cotton locusts and bamboo locusts. In my country alone, migratory locusts and soil locusts occur on an annual basis of more than 25 million mu and 70 million mu respectively, seriously affecting agricultural and animal husbandry production. The current method of controlling locusts is mainly to use chemical pesticides to prevent and control. Although chemical control can quickly reduce the population density of locusts, it often causes adverse consequences such as polluting the environment, killing a large number of beneficial anim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCC12N1/14A01N63/30
Inventor 聂江维
Owner 北京中维新兴技术有限公司
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