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Application of intestinal flora in hepatic encephalopathy detection

A technology for hepatic encephalopathy and Escherichia coli, which is applied to the application field of intestinal flora in the detection of hepatic encephalopathy, can solve problems such as the relationship between intestinal flora that has not been disclosed in the literature, and achieve simple operation and high specificity , the effect of strong sensitivity

Active Publication Date: 2021-04-27
RUIAN PEOPLES HOSPITAL +1
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Problems solved by technology

[0019] Currently, there is no published literature on the relationship between intestinal flora and hepatic encephalopathy

Method used

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  • Application of intestinal flora in hepatic encephalopathy detection
  • Application of intestinal flora in hepatic encephalopathy detection
  • Application of intestinal flora in hepatic encephalopathy detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0049]Example 2 Fluorescence Quantitative PCR Verification Related Flora

[0050]1. Multiple sample QPCR verification is carried out in accordance with the sample collection method in Example 1, 30 cases of feces samples in patients with central hepological encephalopathy were collected.

[0051]2, extraction and quantification of manure bacteria DNA

[0052]Bacteria DNA is extracted from the manure specimens using a fecal genome DNA extraction kit, and the operation step is carried out in accordance with the instructions;

[0053]The total DNA concentration and purity of the genome DNA was determined by 1% agarose gel electrophoresis, and all the mass concentrations of all sample DNA were uniform to 100 mg / L.

[0054]3, real-time fluorescent quantitative PCR

[0055]1) Primer design and synthesis

[0056]According to the V3-V4 sequence of the bacterial 16SrNA gene. Design specific primers and a reference gene in 16SRRNA-general primers, primers were synthesized by Shanghai Freight Biological Engineer...

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Abstract

The invention relates to an application of intestinal flora in hepatic encephalopathy detection. The intestinal flora comprises enterohemorrhagic Escherichia coli, gemella haemolysans and Helcococcus kunziii. The level change of the specific flora in the hepatic encephalopathy is found for the first time, the hepatic encephalopathy can be diagnosed by detecting the change of the specific flora, and when the microbial marker is used for diagnosis, the operation is simple, the specificity is high, and the sensitivity is strong.

Description

Technical field[0001]The present invention belongs to the field of biological detection, and specific to the use of intestinal flora in hepatic encephalopathy test.Background technique[0002]Hepatic cerebral disease (HE) is also known as hepatic coma, which refers to the syndrome of a central nervous system dysfunction caused by a serious liver disease. Its main clinical manifestation is a consciousness, behavioral disorder and coma. Due to the type of liver disease, the degree of hepatocyte damage, the acuteness of the onset, and the difference in incentives. Due to different basic diseases that lead to hepatic encephalopathy, its clinical manifestations are complicated, variable, and variability of early symptoms is the characteristics of this disease. But there is also its common performance: it is reflected in neurological symptoms and signs. There are both primitive liver basic diseases, and there is its unique clinical manifestations, generally manifestative, behaviors, intelli...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/19C12R1/01
CPCC12Q1/689G01N2800/28
Inventor 黄鹏张宏宇龚方华沈秀微王丰伟吴淑娟
Owner RUIAN PEOPLES HOSPITAL
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