Full-automatic microbial nucleic acid extraction and detection device and extraction and detection method

Abstract

The invention relates to a full-automatic microbial nucleic acid extraction and detection device and an extraction and detection method. The device comprises a mechanical arm, a plurality of reagent tubes, a low-temperature plasma generation device, a magnetic needle probe, a heating rod and a liquid guide tube, the mechanical arm comprises a plurality of mechanical arms, and the mechanical arms are connected with ports of the reagent tubes, the low-temperature plasma generation device, the magnetic needle probe and the heating rod in a matched mode; wherein the low-temperature plasma generating device, the magnetic needle probe and the heating rod respectively extend into the reagent tubes in a matching way, the bottoms of the plurality of reagent tubes are respectively connected with the liquid guide tubes, the reagent tubes are movably connected with the liquid guide tubes, and switches are arranged at the joints, so that the reagent tubes are communicated or closed with the liquid guide tubes. The method can be used for extracting and analyzing the nucleic acid of microorganisms in gas, solid and liquid samples in various places, and the working efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of detection devices, and in particular relates to a fully automatic microbial nucleic acid extraction and detection device and an extraction and detection method. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] In nucleic acid detection experiments, obtaining high-purity nucleic acids is one of the most important steps, which is closely related to subsequent application analysis such as gene amplification, sequencing, hybridization, and cloning. Commonly used microbial nucleic acid extraction techniques include phenol-chloroform method, salting out method, silica phase adsorption method, ion exchange met...

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Problems solved by technology

However, due to the large size of these instruments and the need for external power supplies, etc., they are mainly used in laboratory disinfection research at present.

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