The invention relates to an anti-loose internally threaded fastener, which comprises an internal thread substrate, and is characterized in that the shape of an internal thread arranged on the internal thread substrate is as follows: the oblique angle of an internal thread profile is 38 degrees to 40 degrees, the intermediate diameter D2 = D-(0. 42 to 0.43) P, the variable angle inflection point diameter phiD = D-(0.48 to 0.50)P, the minor diameter D1 = D-(0.90 to 0.91) P, the theoretical interference width is 0.12B, the theoretical interference height is 0.22Td, the failure theoretical distance is 0.098P, the intermediate diameter position is 0.215P, and the inflection point position is 0.24P. By improving the design parameters of the thread, and connecting the internal thread with six-level machining precision and an ordinary external thread with six-level machining precision, the mechanical properties of a thread pair are greatly improved, the contact stress of the thread pair is reduced, the plastic deformation of an external thread profile crest zone is reduced, the overloading assembly ability is strong, the anti-loose reliability is good, no interference occurs in a limit size state, the rotation following phenomenon is avoided, and the assembly quality and efficiency are high.