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Method for detecting five cannabinol compounds in cannabis oil by utilizing HPLC method

A technology of cannabinol and compounds, applied in the field of analytical chemistry, can solve problems such as the lack of result verification process, and achieve the effect of simple operation and short time consumption

Pending Publication Date: 2021-04-30
CHINA NAT INST OF STANDARDIZATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In summary, there is no report on the simultaneous analysis and detection of THC, THCA, CBD, CBDA, and CBN in hemp oil, and the existing methods lack a systematic result verification process

Method used

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  • Method for detecting five cannabinol compounds in cannabis oil by utilizing HPLC method
  • Method for detecting five cannabinol compounds in cannabis oil by utilizing HPLC method
  • Method for detecting five cannabinol compounds in cannabis oil by utilizing HPLC method

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Experimental program
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Effect test

Embodiment 1

[0052] Detection method

[0053] 1) Introduction to Materials and Instruments

[0054] Standard cannabinol: CBDA, CBD, CBN, THC, THCA standard (1mg / mL, 1mL), methanol, acetonitrile, isopropanol (chromatographically pure). The five producing areas of hemp oil are produced in Shanxi, Shaanxi, Guangxi, Heilongjiang and Gansu respectively.

[0055] iChrom5100 analytical liquid chromatograph with DAD detector; Supersil-ODS2 chromatographic column (4.6mm×250mm, 5μm); vortex shaker; centrifuge; analytical balance; pure water meter; m-PFC fast filter column.

[0056] 2) Solution configuration

[0057] Mobile phase: mobile phase A is pure water, which is made by a laboratory water purifier, with a conductivity of 18.2 MΩ. Before use, it is filtered through a water phase membrane and degassed by ultrasonic. The mobile phase B was acetonitrile (chromatographically pure), and it was suction-filtered through an organic phase filter membrane and degassed by ultrasonic before use.

[005...

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Abstract

The invention belongs to the field of analytical chemistry, and relates to a method for detecting five cannabinol compounds in cannabis oil by high performance liquid chromatography (HPLC). According to the method, a C18 chromatographic column is adopted, ultrapure water is used as a mobile phase A, acetonitrile is used as a mobile phase B for gradient elution, and a sample enters a detector for detection. The cannabinol compounds are delta 9-tetrahydrocannabinol, cannabidiol, cannabinol, tetrahydrocannabinolic acid and cannabidiolic acid. The technical scheme provided by the invention can lay a methodological foundation for the establishment of related detection method standards, and has important meanings for preventing cannabis oil with overproof cannabinol content from flowing into the market and promoting the effective supervision of industrial cannabis food at home and abroad.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for detecting five cannabinoid compounds in hemp oil by using high performance liquid chromatography (HPLC). Background technique [0002] The main active ingredients in cannabis are cannabinoids, including tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA), cannabinol (CBN), cannabidiol (CBD), △9 -Tetrahydrocannabinol (THC) is the most widely used of these 5 types. [0003] Among the five cannabinoids, tetrahydrocannabinolic acid, cannabidiolic acid, cannabinol, and cannabidiol all have certain pharmacological activities. For example, cannabidiol has medical effects on epilepsy, depression, multiple sclerosis, etc. Diseases have obvious therapeutic effects. and △9 - THC can affect the central nervous system after inhalation or oral administration, and can be transformed with cannabinol and cannabidiol, so it needs to be strictly controlled. ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/7233G01N30/8679G01N2030/146
Inventor 兰韬席兴军云振宇初侨于聪聪孟玲玲张文焕
Owner CHINA NAT INST OF STANDARDIZATION
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