Supercharge Your Innovation With Domain-Expert AI Agents!

Oryza sativa L. rice-blast-resistant gene marker system based on KASP technology, and application of Oryza sativa L. rice-blast-resistant gene marker system

A rice blast resistance gene and rice blast resistance technology is applied in the field of primer combinations for rice blast resistance genotyping, and can solve the problems of complex population structure, frequent pathogenic variation of physiological races, loss of resistance of disease-resistant varieties, and the like , to achieve the effect of simple operation, low cost, accurate and reliable test results

Active Publication Date: 2021-05-04
YUAN LONGPING HIGH TECH AGRI CO LTD +2
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Conventional breeding methods have a long period of breeding disease-resistant varieties, coupled with frequent pathogenic variation of physiological races of Magnaporthe oryzae and complex population structure, the resistance of the bred disease-resistant varieties containing a single resistance gene is relatively fast, which is difficult to achieve. Long-lasting resistance in resistant varieties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oryza sativa L. rice-blast-resistant gene marker system based on KASP technology, and application of Oryza sativa L. rice-blast-resistant gene marker system
  • Oryza sativa L. rice-blast-resistant gene marker system based on KASP technology, and application of Oryza sativa L. rice-blast-resistant gene marker system
  • Oryza sativa L. rice-blast-resistant gene marker system based on KASP technology, and application of Oryza sativa L. rice-blast-resistant gene marker system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Development and Verification of Important Rice Blast Resistance Gene Markers

[0045] Tag development process:

[0046]1. Collect related gene positioning and cloning articles to obtain the mutation sites that affect gene function; if there are no functional mutation sites, obtain the position of the cloned gene on the reference genome, and use NCBI to search for the corresponding gene sequence for the cloned gene. Perform NCBI blast analysis on the gene sequence to obtain gene-specific variation sites, use the 3000 rice resequencing database to analyze the distribution frequency of favorable alleles at gene variation sites, and select genes with extremely low (or none) or rare positions with favorable allelic variation frequencies The point is the marker site;

[0047] 2. For functional gene loci that do not have rare variations within the gene, the second-generation genome-wide resequencing of the gene donor material is performed to obtain the SNP or insert...

Embodiment 2

[0058] Example 2 Application of important rice blast resistance gene marker system in breeding parents and resource identification

[0059] 1. Using 20 rice blast resistance gene KASP marker primer sets, carry out KASP reaction on 32 rice breeding materials or resources, and test the marker typing. The names of 32 materials are shown in Table 3 or Table 4.

[0060] 2. DNA extraction: Genomic DNA was extracted from rice leaves, using the simplified CTAB method.

[0061] 3. KASP genotyping: KASP reaction test is performed in offline mode on the LGC IntelliQube genotyping platform. Add 0.8ul DNA sample to the microwell reaction plate, then add 0.8ul KASP reaction mixture, KASP reaction mixture: 100mM primers are premixed according to the ratio of primers Fam:Hex:Com=1:1:2 , and then add primers to the KASP master mix according to the ratio of primer mixture: KASP master mix = 1:36 to configure the KASP reaction mixture. PCR amplification was performed in a water bath thermal c...

Embodiment 3

[0070] Example 3 Blast Resistance and Resistance Gene Analysis of Longliangyou and Jingliangyou Series Hybrid Rice Combinations

[0071] (1) Test material

[0072] The rice materials are 72 hybrid rice varieties of Longliangyou and Jingliangyou series (accumulated 120 times passed the national examination and approval), see Table 5 for details;

[0073] (2) Rice blast resistance evaluation

[0074] The blast resistance evaluation data of Longliangyou and Yujing Liangyou series hybrid rice combinations in this study are all from the National Rice Data Center (http: / / www.ricedata.cn / variety / index.htm);

[0075] (3) Genotype analysis

[0076] Utilize 20 rice blast resistance gene KASP detection marker combinations of the present invention to analyze the test material, and the specific genotype detection steps are the same as steps 1, 2, and 3 in Example 2;

[0077] (4) Analysis of results

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of molecular biology and crop breeding, in particular to an Oryza sativa L. rice-blast-resistant gene marker system based on a KASP technology, and application of the Oryza sativa L. rice-blast-resistant gene marker system. The invention provides 20 kinds of Oryza sativa L. rice-blast-resistant gene SNP markers, and KASP primer group sequences used for amplifying the SNP markers are disclosed by SEQ ID No.1-60. The KASP primer group provided by the invention can be used for Oryza sativa L. molecular marker auxiliary disease-resistant breeding, a single rice-blast-resistant gene is imported or a plurality of rice-blast-resistant genes are polymerized to realize directional improvement of Oryza sativa L. variety disease resistance or cultivation of a new disease-resistant variety, rice-blast-resistant gene types, amounts and combinations carried by existing Oryza sativa L. varieties and resources can be comprehensively and systematically detected, and data support is provided for efficient utilization of a disease-resistant gene and a donor thereof and a reasonable layout of the Oryza sativa L. variety.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and crop breeding, in particular to a combination of primers for rice blast resistance genotyping based on KASP technology and its application. Background technique [0002] Rice is one of the most important food crops in China and even in the world. More than half of the world's population uses rice as a staple food. Rice blast, known as the cancer of rice, is one of the most serious diseases to rice production. In order to prevent and control rice blast, a large number of fungicides are used in rice production. The use of fungicides has reduced the damage of rice blast to rice production to a certain extent, but it has caused problems such as increased rice production costs and environmental pollution. At the same time, long-term The use of rice blast bacteria is prone to resistance. Practice has proved that cultivating and planting disease-resistant varieties is the most economical...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 杨远柱邓钊王凯傅军严天泽江南刘兰兰石媛媛
Owner YUAN LONGPING HIGH TECH AGRI CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com