Application of diagnostic marker for newborn biliary atresia

A diagnostic marker and biliary atresia technology, applied in the field of biomedicine, can solve the problems of limited accuracy, large differences in children, and many factors affecting platelet measurement, and achieve high accuracy, reliable detection results, and low detection limit. Effect

Pending Publication Date: 2021-05-04
HUMAN METABOLOMICS INST INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

GGT is the most commonly used screening index for biliary atresia, but in clinical cases, there are large differences among different children, and the accuracy is limited; ③Other indexes: serum bile acid, prothrombin concentration, and platelet determination have many influencing factors

Method used

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  • Application of diagnostic marker for newborn biliary atresia
  • Application of diagnostic marker for newborn biliary atresia
  • Application of diagnostic marker for newborn biliary atresia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Embodiment 1 The establishment and optimization of liquid chromatography-tandem mass spectrometry method

[0088] In this example, the establishment process of the qualitative and quantitative detection method for the components of bilirubin in biological samples based on liquid chromatography-tandem mass spectrometry is described for the purpose of illustration, including selection of liquid phase conditions, setting of mass spectrometry conditions, preparation of standard solutions , Optimizing the analysis method, drawing a standard curve. The detection method of this embodiment has high sensitivity and high selectivity, strong anti-interference ability, and can become an effective tool for qualitative and quantitative analysis of bilirubin components.

[0089] Bilirubin samples contain a variety of components. The inventor found that when the pH value of the mobile phase A of liquid chromatography was 3-4.5, the main components in the bilirubin sample were free bil...

Embodiment 2

[0112] Qualitative and quantitative determination of bilirubin in embodiment 2 blood samples

[0113] Sampling: Take the serum, plasma and whole blood samples of 3 test subjects, and each sample is divided into 3 parts, such as serum 01, serum 02, serum 03, etc., a total of 9 biological samples.

[0114] Biological sample processing:

[0115] Serum 01: Take 20 microliters of samples, add 10 microliters of bilirubin taurine as an internal standard, and then add 80 microliters of methanol:acetonitrile (volume ratio 1:1) solution containing 1mg / ml BHT and 200mmol / L ascorbic acid , Shake at 1450 rpm for 20 minutes at 10°C in the dark. Centrifuge at 18,000 g for 20 minutes at 4°C. Take 60 microliters of supernatant to a 96-well plate, and use it as a detection sample for high performance liquid chromatography tandem mass spectrometry detection.

[0116] Serum 02: Take 20 microliters of samples, add 10 microliters of bilirubin taurine as an internal standard, then add 80 microlit...

Embodiment 3

[0128] Qualitative and quantitative determination of bilirubin components in embodiment 3 dried blood spots

[0129] Dried blood spot sample preparation

[0130] Fingertip blood was collected from newborns within 4 days of birth to prepare dry blood spot samples. Proceed as follows:

[0131] a) Pretreatment of filter paper: Soak 903 filter paper in ethanol solution containing 1mg / mL BHT and 200mmol / L ascorbic acid repeatedly for 2 minutes, take it out and drain, then dry the filter paper naturally in the shade for later use.

[0132] b) Preparation: Take a suitable test tube and add an appropriate amount of diluent. Take a micropipette and connect it with a latex tip, check whether there is any air leakage at the connection, or take a disposable micropipette (siphon principle), blood collection needle, 75% ethanol or iodophor, cotton swab, etc. for backup.

[0133] c) Massage: Gently massage the central part of the ulnar side of the ring finger of the left hand with more mu...

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Abstract

The invention discloses application of a newborn biliary atresia diagnostic marker in preparation of a tool for evaluating and diagnosing newborn biliary atresia. The diagnostic product can be a medical instrument, a kit, test paper, a medical device and the like. The invention also provides a kit for detecting the biomarker and a use method of the kit. The invention provides an effective means for early screening of the risk of neonatal biliary atresia and diagnosis different from cholestasis caused by other reasons, improves the diagnosis efficiency and diagnosis accuracy of the neonatal biliary atresia, strives for time for taking treatment measures in time, and is beneficial to improving the treatment effect and prognosis of the neonatal biliary atresia.

Description

technical field [0001] The present invention relates to the field of biomedical technology, in particular to a diagnostic marker that can be used to assess the risk of neonatal biliary atresia and its application in the field of monitoring, screening and diagnosis of neonatal biliary atresia, and the present invention also relates to the detection of the diagnostic marker Methods and Apparatus. Background technique [0002] Biliary atresia (BA) is a progressive, idiopathic extrahepatic biliary system disease that occurs in the neonatal period and is a devastating neonatal inflammatory fibrous obstruction of the bile ducts affecting intrahepatic and extrahepatic bile ducts of varying lengths, It is the most common cause of neonatal obstructive jaundice and the most common cause of pediatric liver transplantation. About 60% of children's liver transplantation is caused by this. If children diagnosed with congenital biliary atresia are not treated in time, more than 80% will d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72G01N30/88G01N33/52
CPCG01N33/728G01N30/88G01N33/52G01N2800/085G01N2800/38
Inventor 贾伟谢国祥周科军
Owner HUMAN METABOLOMICS INST INC
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