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Quantum dot-based FRET fluorescent dye and application thereof

A fluorescent dye and quantum dot technology, applied in the application of the fluorescent dye, based on the quantum dot FRET fluorescent dye field, can solve the problems of low fluorescence quantum efficiency, weak fluorescence intensity, and susceptibility to background interference

Active Publication Date: 2021-05-07
北京旌准医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fluorescence quantum efficiency of this kind of material itself is low, the fluorescence intensity is weak, and it is easy to be interfered by the background, thus affecting its detection sensitivity.

Method used

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  • Quantum dot-based FRET fluorescent dye and application thereof
  • Quantum dot-based FRET fluorescent dye and application thereof
  • Quantum dot-based FRET fluorescent dye and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Synthesis of Example 1 Compound 7 ddATP-Bodipy FL

[0038] (1) Synthesis of Compound 3 Bodipy FL-EDA-Boc

[0039] Take a 5ml clean double-mouthed round bottom reaction bottle, add a magnetic stirrer, add 10mg of raw material 1 Bodipy-FLNHSEster, seal it, avoid light, and protect it under nitrogen. Take 10 microliters of N,N-diisopropylethylamine and dissolve it in 1 milliliter of anhydrous N,N-dimethylformamide (DMF), then inject the solution into the reaction bottle and stir well until the raw material 1 is completely dissolved , and then add 2100 microliters of the compound at one time, and react at room temperature for 2 hours in the dark. After the reaction, the reaction solution was added dropwise to a mixed solution of acetone / petroleum ether with a volume ratio of 1:1 to precipitate the product, and centrifuged to obtain a solid product with a yield of 92%.

[0040] (1) Synthesis of Compound 4 Bodipy FL-EDA

[0041]The product 3 obtained in (1) was added to a ...

Embodiment 2

[0046] The synthesis steps of compound ddUTP-Bodipy 550 in Example 2 are similar to the synthesis steps of compound 7 ddATP-Bodipy FL.

Embodiment 3

[0047] The synthesis steps of Example 3 compound ddCTP-Rhodamine are similar to the synthesis steps of compound 7 ddATP-Bodipy FL.

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Abstract

The invention provides a quantum dot-based FRET fluorescent dye. The quantum dot-based FRET fluorescent dye comprises a quantum dot subunit, an organic fluorescent dye subunit, a linking group subunit and a biological binding site subunit. According to the fluorescent dye, quantum dots are used as donor groups to absorb excitation light energy, the energy is transferred to acceptor fluorophores through intramolecular fluorescence resonance energy transfer (FRET), excitation light with different wavelengths is excited, and therefore excitation with one excitation wavelength and multi-color fluorescence detection are achieved. The surface of the quantum dot is connected with a plurality of organic fluorescent dye molecules, so the effect of amplifying a fluorescent signal can be realized. The invention also provides application of the fluorescent dye.

Description

technical field [0001] The invention relates to the field of molecular diagnosis, in particular to a fluorescent dye based on quantum dot FRET and the application of the fluorescent dye. Background technique [0002] Quantum dots are semiconductor nanostructures that can confine excitons in three dimensions. The principle of luminescence is that when the particle size of quantum dots is smaller than or close to the Bohr radius of excitons, it limits the movement of excitons in three-dimensional space, thereby producing a discontinuous electronic energy level structure similar to atoms, and electrons and holes are quantum-confined. Domains, after being excited, can emit light of different wavelengths with different sizes and atomic structures. Compared with organic light-emitting materials, quantum dot materials have the characteristics of easy adjustment of wavelength range, wide absorption, narrow emission, large Stokes shift, small filter loss, strong anti-bleaching abili...

Claims

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Application Information

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IPC IPC(8): C09B57/00C09K11/06G01N21/64
CPCC09K11/06C09B57/00G01N21/6428C09K2211/1044C09K2211/1088C09K2211/1059G01N2021/6439
Inventor 赵子健吴志鸿左杰叶锋
Owner 北京旌准医疗科技有限公司