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Application of talaromyces wortmannii in drought resistance and growth promotion of crops

A basket-shaped fungus and crop technology, applied in the field of plant drought resistance, can solve problems such as not very significant effects, affecting plant growth and development, crop production and income reduction, etc., achieve good development and application prospects, facilitate large-scale production, and increase growth speed Effect

Pending Publication Date: 2021-05-07
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing technology has the following deficiencies: drought and early adversity will seriously affect the growth and development of plants, resulting in reduced crop production and income. At present, seaweed fertilizers, amino acid water-soluble fertilizers, and plant growth regulators are mainly used in the agricultural field to assist crops in drought resistance, but the effect is not good. Not very significant, especially for crops that are susceptible to drought stress, such as corn, rice, etc. Therefore, improving the drought resistance of seeds through genetic engineering is of great significance for the reduction in yield caused by drought

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The application of a Talaromyces wortmannii provided by the invention in crop drought resistance and growth promotion comprises the following specific steps:

[0026] S1 Preparation: Add 30mL of PDB culture medium into a sterile centrifuge tube, then punch 18 pieces of bacteria and put them in a sterile centrifuge tube, culture them in a constant temperature shaking incubator for 3 days to make a bacteria solution, and count them with a hemocytometer The number of spores to make it ferment, the culture condition in the constant temperature shaking incubator is 28 ℃, 200rmp, the number of spores in the fermentation liquid needs to be adjusted to 106CFU / ml when the bacterial liquid is prepared, so as to ensure the stable fermentation environment of the bacterial liquid and lay a good foundation for the seeds. Growth base.

[0027] S2 Seed soaking: Select healthy seeds with full particles for surface disinfection, and then soak the seeds in the bacterial fermentation broth...

Embodiment 2

[0034] The application of a Talaromyces wortmannii provided by the invention in crop drought resistance and growth promotion comprises the following specific steps:

[0035] S1 Preparation: Add 30mL of PDB culture medium into a sterile centrifuge tube, then punch 18 pieces of bacteria and put them in a sterile centrifuge tube, culture them in a constant temperature shaking incubator for 4 days to make a bacterial solution, and count them with a hemocytometer The number of spores to make it ferment, the culture condition in the constant temperature shaking incubator is 28 ℃, 200rmp, the number of spores in the fermentation liquid needs to be adjusted to 106CFU / ml when the bacterial liquid is prepared, so as to ensure the stable fermentation environment of the bacterial liquid and lay a good foundation for the seeds. Growth base.

[0036]S2 Seed soaking: Select healthy seeds with full particles for surface disinfection, and then soak the seeds in the bacterial fermentation broth...

Embodiment 3

[0043] The application of a Talaromyces wortmannii provided by the invention in crop drought resistance and growth promotion comprises the following specific steps:

[0044] S1 Preparation: Add 30mL of PDB culture medium into a sterile centrifuge tube, then punch 18 pieces of bacteria and put them in a sterile centrifuge tube, culture them in a constant temperature shaking incubator for 5 days to make a bacterial solution, and count them with a hemocytometer The number of spores to make it ferment, the culture condition in the constant temperature shaking incubator is 28 ℃, 200rmp, the number of spores in the fermentation liquid needs to be adjusted to 106CFU / ml when the bacterial liquid is prepared, so as to ensure the stable fermentation environment of the bacterial liquid and lay a good foundation for the seeds. Growth base.

[0045] S2 Seed soaking: Select healthy seeds with full particles for surface disinfection, and then soak the seeds in the bacterial fermentation brot...

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PUM

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Abstract

The invention discloses an application of talaromyces wortmannii in drought resistance and growth promotion of crops. The technical scheme is as follows: the application comprises the following specific steps: S1, preparation, S2, seed soaking, S3, sowing, S4, water removing, S5, statistics, S6, study on a drought-enduring mechanism of a strain, and S7, polygene sequence analysis. The application has the beneficial effects that by means of manual regulation and control in a laboratory, seeds to be identified are soaked, germinate and grow to a specific seedling height under a temperature control condition, temperature control and humidity control treatment are performed by utilizing a temperature control and humidity control illumination greenhouse, so that seedlings growing on potted plants lose water, drought-enduring treatment and repair treatment can be performed on the seedlings, and the growth conditions of roots, leaves and the like of the seedlings are observed, so that drought resistance of crops is judged. The talaromyces wortmannii has obvious effects in the aspects of improving the growth speed and drought resistance of the crops and the like, can be artificially cultured, is high in propagation speed, is convenient for large-scale production, and has a good development and application prospect.

Description

technical field [0001] The invention relates to the technical field of plant drought resistance, in particular to the application of Talaromyceswortmannii in crop drought resistance and growth promotion. Background technique [0002] The shortage of water resources is a global problem currently restricting agricultural production. With the rapid development of industrialization, frequent natural disasters, and the reduction of precipitation, about 43% of the world's arable land is dry and semi-arid. It poses a huge challenge. Rhizosphere microorganisms are the general term for microorganisms distributed in the rhizosphere soil of crops. The microorganisms active in the rhizosphere soil are closely related to the growth status of crops. Talaromyces wortmannii, with the strain number MN122989, has been preserved in the Guangdong Microbial Culture Collection Center. [0003] The existing technology has the following deficiencies: drought and early adversity will seriously affe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/686A01G22/20A01G22/22A01G7/06C12Q1/04C12R1/645
CPCC12Q1/6895C12Q1/686A01G22/20A01G22/22A01G7/06
Inventor 陈泽怡张颖施陈娟
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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