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Colloidal gold immunochromatography trace whole blood detection test paper and detection method thereof

A technology of immunochromatography and detection test strips, which is applied in biological testing, measuring devices, and analysis materials, etc., and can solve the problems of peripheral blood sampling trauma, increased operability, and pain of sampling people

Active Publication Date: 2021-05-07
CHENGDU YONGAN PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]In the traditional testing process, the test sample of blood sample is often more than 50μL, if the peripheral blood is used to take 50μL or more, it often squeezes the peripheral part and causes the pain of the sampler
However, taking venous blood requires professional nurses, which increases the trauma and operability of peripheral blood sampling.

Method used

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  • Colloidal gold immunochromatography trace whole blood detection test paper and detection method thereof
  • Colloidal gold immunochromatography trace whole blood detection test paper and detection method thereof
  • Colloidal gold immunochromatography trace whole blood detection test paper and detection method thereof

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: A kind of colloidal gold immunochromatography trace whole blood detection test paper, structure such as figure 1 As shown, it is composed of PVC bottom plate 1, glass cellulose membrane 2, gold bonding pad 3, T line 6, C line 7, nitrocellulose membrane 4, micro whole blood filter membrane) and absorbent paper 5, and the glass fiber The plain film 2 is located on the left side of the PVC base plate 1, the absorbent paper 5 is located on the right side of the PVC base plate 1, the nitrocellulose membrane 4 is located on the PVC base plate 1, and the right side of the gold bonding pad 3 is located on the left side of the upper surface of the nitrocellulose membrane 4. side, the left side of the gold bonding pad 3 extends to the bottom right of the glass cellulose membrane 2, the left side of the absorbent paper 5 is located on the right side of the upper surface of the nitrocellulose membrane 4, and the upper surface of the nitrocellulose membrane 4 extends fr...

Embodiment 2

[0033] Embodiment 2: A kind of colloidal gold immunochromatography trace whole blood detection test paper, structure such as figure 1 As shown, it is composed of PVC bottom plate 1, glass cellulose membrane 2, gold bonding pad 3, T line 6, C line 7, nitrocellulose membrane 4, micro whole blood filter membrane) and absorbent paper 5, and the glass fiber The plain film 2 is located on the left side of the PVC base plate 1, the absorbent paper 5 is located on the right side of the PVC base plate 1, the nitrocellulose membrane 4 is located on the PVC base plate 1, and the right side of the gold bonding pad 3 is located on the left side of the upper surface of the nitrocellulose membrane 4. side, the left side of the gold bonding pad 3 extends to the bottom right of the glass cellulose membrane 2, the left side of the absorbent paper 5 is located on the right side of the upper surface of the nitrocellulose membrane 4, and the upper surface of the nitrocellulose membrane 4 extends fr...

Embodiment 3

[0042] Embodiment 3: A kind of colloidal gold immunochromatography trace whole blood detection test paper, structure such as figure 1 As shown, it is composed of PVC bottom plate 1, glass cellulose membrane 2, gold bonding pad 3, T line 6, C line 7, nitrocellulose membrane 4, micro whole blood filter membrane) and absorbent paper 5, and the glass fiber The plain film 2 is located on the left side of the PVC base plate 1, the absorbent paper 5 is located on the right side of the PVC base plate 1, the nitrocellulose membrane 4 is located on the PVC base plate 1, and the right side of the gold bonding pad 3 is located on the left side of the upper surface of the nitrocellulose membrane 4. side, the left side of the gold bonding pad 3 extends to the bottom right of the glass cellulose membrane 2, the left side of the absorbent paper 5 is located on the right side of the upper surface of the nitrocellulose membrane 4, and the upper surface of the nitrocellulose membrane 4 extends fr...

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Abstract

The invention discloses a colloidal gold immunochromatography trace whole blood detection test paper and a detection method thereof. A sample is added at the front end and is different from a traditional back-end sample adding technology, meanwhile, a trace whole blood filter membrane is additionally arranged on a front-end nitrocellulose membrane, and after the trace whole blood is added, the trace whole blood passes through the treated whole blood filter membrane, serum permeates into the nitrocellulose membrane, red blood cells and the like are retained on the whole blood filter membrane, and the effect of rapidly separating the red blood cells is achieved. The application range and the detection effect of the trace whole blood are widened, and meanwhile the test paper can also be used for serum and plasma; and according to the method, peripheral blood collection is very easy, trauma is small, experience of a blood supplier is also easy, the detection method is simple, convenient and rapid, and results are accurate and reliable.

Description

technical field [0001] The invention belongs to the field of quarantine inspection and testing, and in particular relates to a colloidal gold immune chromatography trace whole blood detection test paper and a detection method thereof. Background technique [0002] The basic principle of immune colloidal gold technology is: chloroauric acid can be polymerized into gold particles of a certain size under the action of a reducing agent to form a hydrophobic colloidal solution. It becomes a stable colloidal state due to electrostatic interaction, so it is called colloidal gold. Colloidal gold labeling is essentially a coating process in which polymers such as proteins are adsorbed to the surface of colloidal gold particles. Its adsorption principle is generally believed to be due to the electrostatic interaction between the negative charges on the surface of gold particles and the positively charged groups of the protein - the van der Waals attraction of non-covalent bonds, and ...

Claims

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Application Information

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IPC IPC(8): G01N33/80G01N33/58G01N33/558G01N33/543
CPCG01N33/80G01N33/558G01N33/585G01N33/543
Inventor 叶成栋刘军
Owner CHENGDU YONGAN PHARMA CO LTD
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