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Preparation method of paracrine factor inclusion with skin cell rejuvenation effect

A skin cell and paracrine technology, which is applied to skin care preparations, medical preparations containing active ingredients, cosmetic preparations, etc., can solve the problems of inconvenient use of paracrine factors, and achieve increased division activity and increased growth rate. , the effect of prolonging life

Pending Publication Date: 2021-05-11
朱瑜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for preparing paracrine factor inclusions with the effect of skin cell rejuvenation, which has the advantages of easy use of paracrine factor inclusions and solves the problem of inconvenient use of paracrine factors

Method used

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Examples

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Effect test

preparation example Construction

[0018] A method for preparing paracrine factor inclusions with the effect of rejuvenating skin cells, comprising the following steps:

[0019] Step a: placing the umbilical cord in a storage and transportation bottle containing a storage and transportation solution, to be separated and prepared;

[0020] Step b: Put the umbilical cord in a petri dish, wash the umbilical cord fully with normal saline, cut off 1 cm at both ends of the umbilical cord, and then repeatedly wash the umbilical cord tissue until there is no obvious blood clot, cut the umbilical cord into small pieces with a length of 2 cm, and wash for 3 First, cut the umbilical cord longitudinally, remove blood vessels, tear off Wharton’s jelly, wash Wharton’s jelly three times with normal saline, and then cut it into pieces of tissue with a size of 1 mm3 to 3 mm3. Inoculate in a culture bottle, place it in an incubator at 37°C and 5% CO2 for 2 hours, add 15ml of serum-free complete medium, place it in the incubator,...

Embodiment 1

[0025] The method for preparing paracrine factor inclusions with the effect of rejuvenating skin cells comprises the following steps: step a: placing the umbilical cord in a storage and transportation bottle containing a storage and transportation solution to be separated and prepared; step b: placing the umbilical cord in a culture dish , fully wash the umbilical cord with normal saline, cut off 1 cm at both ends of the umbilical cord, and then repeatedly wash the umbilical cord tissue until there is no obvious blood clot, cut the umbilical cord into small pieces with a length of 2 cm, wash 3 times, cut the umbilical cord longitudinally, and remove blood vessels , tear off Wharton's jelly, wash Wharton's jelly three times with normal saline, and then cut it into pieces of 1 cubic millimeter in size, inoculate the tissue pieces in culture bottles, and place them at 37°C in 5% Adhere to the wall in a CO2 incubator for 2 hours, add 15ml of serum-free complete medium, place in the...

Embodiment 2

[0027] In Example 1, the following steps are added:

[0028] In step a, the storage liquid is 40ml of α-MEM, and gentamicin 100U / ml, penicillin 50mg / ml, and amphotericin B 5ug / m are added.

[0029] The method for preparing paracrine factor inclusions with the effect of rejuvenating skin cells comprises the following steps: step a: placing the umbilical cord in a storage and transportation bottle containing a storage and transportation solution to be separated and prepared; step b: placing the umbilical cord in a culture dish , fully wash the umbilical cord with normal saline, cut off 1 cm at both ends of the umbilical cord, and then repeatedly wash the umbilical cord tissue until there is no obvious blood clot, cut the umbilical cord into small pieces with a length of 2 cm, wash 3 times, cut the umbilical cord longitudinally, and remove blood vessels , tear off Wharton's jelly, wash Wharton's jelly three times with normal saline, and then cut it into pieces of 2 cubic millimet...

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Abstract

The invention discloses a preparation method of a paracrine factor inclusion with a skin cell rejuvenation effect. The preparation method comprises the following steps of: step a, putting an umbilical cord into a storage and transportation bottle containing a storage and transportation liquid for later separation and preparation; b, placing the umbilical cord into a culture dish, fully washing the umbilical cord with normal saline, cutting off 1cm of the two ends of the umbilical cord, repeatedly washing the umbilical cord tissue until no obvious blood clots exist, cutting the umbilical cord into small sections with the length of 2cm, washing for three times, longitudinally splitting the umbilical cord, removing blood vessels, tearing off Wharton's jelly, fully washing the Wharton's jelly with normal saline for three times, and then cutting the tissue into tissue blocks with the size of 1-3 cubic millimeters. According to the method, the paracrine factors of the mesenchymal stem cells are wrapped with the microcapsules, can be stored at normal temperature and have stable properties, and the microcapsule inclusion and the fibroblasts are co-cultured, so that the increase of collagen secreted by the fibroblasts can be improved, the division activity can be improved, and the service life of the fibroblasts can be prolonged.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing paracrine factor inclusions with the effect of rejuvenating skin cells. Background technique [0002] Mesenchymal stem cells are a type of pluripotent stem cells derived from mesoderm and ectoderm in early development. MSCs have the functions of immune regulation, promoting the homing of hematopoietic stem cells, repairing damaged or diseased tissues and organs, and possessing multidirectional differentiation potential. It has broad application prospects. [0003] With the deepening of research on mesenchymal stem cells, its mechanism of action is becoming clearer and clearer. There are three main aspects: 1. Paracrine effect of mesenchymal stem cells; 2. Mesenchymal stem cells replace or repair dead or damaged cells 3. Cells directly contact and regulate cells. More and more studies have shown that the paracrine effect of mesenchymal stem cells plays a major ...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/11A61Q19/08
CPCA61K8/99A61K8/11A61Q19/08A61K2800/56
Inventor 朱瑜
Owner 朱瑜
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