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Method of preparing a microscope for imaging a sample

A microscope and sample technology, applied in the field of microscopy, can solve problems such as problems with the correction ring of the objective lens

Pending Publication Date: 2021-05-11
CARL ZEISS MICROSCOPY GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the selection of the correct setting of the objective correction collar remains problematic, especially in the case of varying microscopy tasks and samples

Method used

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  • Method of preparing a microscope for imaging a sample
  • Method of preparing a microscope for imaging a sample
  • Method of preparing a microscope for imaging a sample

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Embodiment Construction

[0044] figure 1 A microscope 2 for imaging a sample 4 is schematically shown. The sample 4 is located on a sample carrier 6 and embedded in an embedding medium 8 below a cover slip 10 . The sample 4, the sample carrier 6, the embedding medium 8 and the cover slip 10 are not part of the microscope, but they are a routine presence in the sample imaging process. Microscope 2 images sample 8 along optical axis 18 onto detector 16 via objective 12 and tube lens 14 . In this case, an immersion medium 19 , usually realized in fluid form, is usually located between the end of the objective 12 and the cover glass 10 . The immersion medium increases optical resolution because it brings about an increase in numerical aperture.

[0045] The objective lens 12 is designed for specific conditions of the sample and a specific focus position, and is especially optimized for spherical aberration. This design is not suitable for different conditions (deviating conditions). For adaptation, ...

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Abstract

A method for preparing a microscope (2) for imaging a sample (4) is provided. The method includes the following steps: providing the microscope for imaging the sample, wherein the microscope comprises an objective having a motor-adjustable objective correction ring (20) for correcting imaging aberrations; acquiring sample information comprising at least one of the following indications: thickness of a cover glass (10), material of the cover glass (10), sample temperature, sample type, sample location on a sample carrier (6), embedding medium (8) of the sample, or immersion medium (19); imaging the sample using the microscope for generating at least one raw image of the sample with a first setting of the objective correction ring (20); and inputting the at least one raw image and the sample information into a machine algorithm (32) and determining a second setting of the objective correction ring, which reduces the imaging aberrations relative to the first setting, by means of the algorithm on the basis of the raw image and the sample information.

Description

technical field [0001] The invention relates to a method for preparing a microscope for imaging a sample, wherein the microscope comprises an objective having an adjustable, in particular motor adjustable, objective for correction of imaging aberrations Correction ring. The invention also relates to microscopic methods. Background technique [0002] Microscope objectives are designed for optimal imaging under specific optical conditions. As resolution increases, the microscope's optical system becomes increasingly sensitive to deviations from the conditions on which it was designed. Image aberrations increase. Therefore, high-performance objectives, especially immersion objectives, have so-called correction collars which act optically on the objective and correct spherical image aberrations which may be caused, for example, by deviating design conditions caused by. Such deviations may be, for example, varying coverslip thicknesses, deviating sample temperatures or tempe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/02G02B7/00G06T7/00
CPCG02B21/02G02B7/00G06T7/0002G06T2207/10056G06T2207/20081G06T2207/20084G06T2207/30168G02B21/365G02B21/06G02B27/0025
Inventor 曼努埃尔·安索丹尼尔·哈泽托马斯·奥特
Owner CARL ZEISS MICROSCOPY GMBH
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