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Paenibacillus polymyxa strain capable of preventing and treating root rot of salvia miltiorrhiza and application of paenibacillus polymyxa strain

A polymyx spore-like and root rot technology, applied in the field of microorganisms, can solve problems such as reducing the yield of Salvia miltiorrhiza, and achieve the effects of environmental friendliness, reduced incidence, and good inhibitory effect.

Inactive Publication Date: 2021-05-14
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Danshen root rot is the most important disease in the production of Danshen, which seriously reduces the yield of Danshen

Method used

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  • Paenibacillus polymyxa strain capable of preventing and treating root rot of salvia miltiorrhiza and application of paenibacillus polymyxa strain
  • Paenibacillus polymyxa strain capable of preventing and treating root rot of salvia miltiorrhiza and application of paenibacillus polymyxa strain
  • Paenibacillus polymyxa strain capable of preventing and treating root rot of salvia miltiorrhiza and application of paenibacillus polymyxa strain

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Embodiment Construction

[0017] The present invention will be further described below in conjunction with the embodiments and the accompanying drawings.

[0018] (1) Separation of biocontrol bacteria

[0019] Weigh 1g of soil, add 10ml of sterile water and shake fully to obtain a soil suspension, then shake the soil suspension evenly and then dilute, take 200μL of the diluted solution and spread it on an LB solid plate, cultivate until the colony grows, pick the size Colonies with different colors or shapes were separated and purified by streaking on the surface of LB solid medium, and a strain was obtained, which was named Pp7-1.

[0020] (2) Screening of biocontrol bacteria (plate antagonism test)

[0021] Using the plate confrontation method to screen biocontrol bacteria, the pathogenic bacteria of root rot of Salvia miltiorrhiza, Fusarium solani, Fusarium oxysporum, and Alternaria tenosporum were respectively inoculated on PDA medium, at 28°C Cultivate 5d, use the puncher that the diameter of st...

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Abstract

The invention discloses a paenibacillus polymyxa strain capable of preventing and treating root rot of salvia miltiorrhiza. The strain is numbered as paenibacillus polymyxa Pp7-1 and is preserved in China Center for Type Culture Collection (CCTCC), Wuhan University, Bayi Road, Hongshan District, Wuhan City, Hubei Province, China, on December 21, 2020, with an accession number being CCTCC M 2020930. The paenibacillus polymyxa strain has a good inhibition effect on pathogenic bacteria of the root rot of the salvia miltiorrhiza, the prevention efficiency on the root rot of the salvia miltiorrhiza reaches 41% or above, the morbidity of the root rot of the salvia miltiorrhiza can be effectively reduced, and the effect of the paenibacillus polymyxa strain is superior to the effect of existing common chemical pesticides. After the paenibacillus polymyxa strain screened by the invention is used, common chemical pesticide problems such as pesticide resistance, pesticide residues and environmental pollution are not generated, and the paenibacillus polymyxa strain is healthy to people and livestock, is friendly to environment and meets the requirements of people on ecological agriculture.

Description

technical field [0001] The invention relates to Paenibacillus polymyxa capable of preventing and treating root rot of salvia miltiorrhiza and its application, belonging to the technical field of microorganisms. Background technique [0002] Salvia miltiorrhizaBge is a perennial herb of the family Lamiaceae. It is used as medicine with dried roots and rhizomes. It is one of the first-selected drugs for the treatment of cardiovascular and cerebrovascular diseases. In recent years, planting Salvia miltiorrhiza has become an important measure for poverty alleviation and rural revitalization in the impoverished mountainous areas of western Henan in Henan Province. In order to ensure the yield of salvia miltiorrhiza, most medicinal planters widely use chemical fertilizers and chemical agents in the process of planting salvia miltiorrhiza, which reduces the quality of salvia miltiorrhiza to a certain extent. In severe cases, fertilizer damage and phytotoxicity may occur, and even l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/12
CPCA01N63/22C12N1/20
Inventor 王飞杨瑾高素霞文艺秦艳红戚文平鲁传涛李雪梦刘玉霞
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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