Composition for promoting absorption of phytochemicals
A phytochemical and absorption-promoting technology, which can be used in drug combination, food science, application, etc., and can solve the problems of undisclosed lactic acid bacteria phytochemicals
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[0071] In the following examples, the following strains were used for comparison.
[0072] Lactobacillus delbrueckii subsp. bulgaricus OLL1247
[0073] The strain was deposited on March 6, 2014 (preservation date) at the National Institute of Technology Evaluation, Patent Microbiology Deposit Center (NPMD) (Room 122, 2-5-8 Kamizu, Kisarazu City, Chiba Prefecture, Japan) with the deposit number NITE BP -01814 made an international deposit based on the Budapest Treaty.
[0074] Lactobacillus delbrueckii subsp. bulgaricus OLL1224
[0075] The strain was deposited internationally under the Budapest Treaty at the National Institute for Technology Evaluation, Patent Microorganisms Depository (NPMD) under the deposit number NITE BP-778 on July 2, 2009 (deposit date).
[0076] Streptococcus thermophilus OLS3078
[0077] The strain was deposited internationally under the Budapest Treaty under the deposit number NITE BP-01697 at the National Institute of Technology Evaluation, ...
experiment example 1
[0097] Experimental example 1: Absorption enhancement of β-carotene (comparison between OLL1251 and other strains)
[0098] (1) Preparation of yogurt
[0099] Lactobacillus bulgaricus OLL1251, Lactobacillus bulgaricus OLL1224, and Lactobacillus bulgaricus OLL1247 were respectively inoculated in a medium containing 10% by mass of skimmed milk powder and 0.5 mM sodium formate, and then the medium was fermented at 43° C. to pH 4.6 and heated. The yogurt thus obtained contained 136 μg / g, 88 μg / g, and 68 μg / g of polysaccharides, respectively.
[0100] (2) Experimental method
[0101] After acclimating 32 rats (SD, male, 8 weeks old, Japan SLC, Inc.) for 7 days, these rats were divided into groups of 8. After 16 hours of fasting, the rats in each group were given β-carotene, β-carotene and yogurt (Lactobacillus bulgaricus OLL1251), β-carotene and yogurt (Lactobacillus bulgaricus OLL1224 ), beta-carotene and yogurt (Lactobacillus bulgaricus OLL1247). Here, β-carotene was admini...
experiment example 2
[0110] Experimental Example 2: Absorption promotion of β-carotene (Streptococcus thermophilus OLS3290 alone)
[0111] (1) Preparation of yogurt
[0112] After inoculating Streptococcus thermophilus OLS3290 and Streptococcus thermophilus OLS3078 in a medium containing 10% by mass of skimmed milk powder and 0.1% by weight of casein peptide (manufactured by DOMO), the medium was fermented at 43°C to pH 4. 6 and heat up. The yogurt thus obtained contained 76.3 μg / g and 45.8 μg / g of polysaccharides, respectively.
[0113] (2) Experimental method
[0114] After acclimating 24 rats (SD, male, 8-week-old, Japan SLC, Inc.) for 7 days, these rats were divided into groups of 8. After 16 hours of fasting, the rats in each group were given β-carotene, β-carotene and yogurt (Streptococcus thermophilus OLS3290), β-carotene and yogurt (Streptococcus thermophilus) respectively. cocci OLS3078). Here, β-carotene was administered at 5 mg / kg body weight, and yoghurt was administered at 11.3...
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