Nicotinamide ribose phosphate transferase for preparing NMN

Abstract

The invention discloses a nicotinamide ribose phosphate transferase for preparing NMN. The nicotinamide ribose phosphate transferase is characterized by comprising an enzyme, wherein the enzyme is an amino-acid-sequence-formed protein which is subjected to substitution, deletion or addition by one or more amino acids or a protein derivative which has activity for catalyzing nicotinamide to obtain beta-nicotinamide mononucleotide. Further, the nicotinamide ribose phosphate transferase also comprises a recombinant expression vector, wherein the recombinant expression vector contains a gene of the nicotinamide ribose phosphate transferase for preparing the NMN. Further, the nicotinamide ribose phosphate transferase also comprises a genetic-engineered host cell, wherein the host cell contains the recombinant expression vector. The process for preparing the beta-nicotinamide mononucleotide NMN, disclosed by the invention, is simple and is short in consumed time, the nicotinamide ribose phosphate transferase employed for preparing the NMN is relatively high in activity and can be used for converting a substrate nicotinamide into the NMN at a conversion ratio of 60% or more at a room temperature, reaction conditions are relatively simple and easily achieved, and the production efficiency of the NMN will be increased relatively after the activity of the enzyme is improved.

Description

technical field [0001] The invention relates to the technical field of biotechnology, in particular to a nicotinamide phosphoribosyltransferase used for preparing NMN. Background technique [0002] NMN is a substance inherent in the human body and is also rich in some fruits and vegetables. In the human body, NMN is the precursor of NAD+, and its function is reflected by NAD+. NAD+ is also called coenzyme, and its full name is nicotinamide adenine dinucleotide. It exists in every cell and participates in thousands of reactions. NMN has important physiological functions for human cells. It can be synthesized naturally in cells and can also be derived from a variety of foods. , including broccoli, cabbage, cucumber, edamame, avocado, and more. In the human body, NMN is the precursor for the synthesis of NAD+, and its physiological function is mainly reflected by increasing the level of NAD+. NAD+ is also called coenzyme Ⅰ, and its full name is nicotinamide adenine dinucleot...

AI Technical Summary

Problems solved by technology

[0004] The traditional production of NMN is chemical synthesis, using nicotinamide ribose as raw material, phosphorylation with phosphorus oxychloride. However, the phosphorylation specificity of chemical synthesis is not high, resulting in too many impurities in the product, separation and purification are extremely difficult, and the overall yield At the same time, the use of organic solvents is large, and the environmental pollution is serious. For example, in 2002, Tanimori et al. used acetyl-protected ribose and nicotinamide to undergo a condensation reaction under the catalysis of TMSOTf; for example, in 2004, Palmarisa et al. used silane Niacinamide is silanized by chemical reagents. Therefore, NMN needs to be prepared by enzymatic method, in which nicotinamide phosphoribosyltransferase is the rate-limiting enzyme of the whole reaction, but the activity of existing nicotinamide phosphoribosyltransferase is too low , making the production efficiency of NMN unsatisfactory

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