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Biomarker related to oral squamous carcinoma

A biomarker, oral squamous cell carcinoma technology, applied in biochemical equipment and methods, microbial determination/examination, drug combination, etc., can solve the problem of low overall survival rate of OSCC patients

Active Publication Date: 2021-05-18
XIANGYA STOMATOLOGICAL HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, the main means of OSCC treatment are surgery, chemotherapy, radiotherapy, or a combination of these treatments. Although significant progress has been made in combined therapy, the overall survival rate of OSCC patients is still very low (Chen YL, Liu KJ, Jang CW, et al. al.ERK ActivationModulates Cancer Sternness and Motility of a Novel Mouse Oral Squamous CellCarcinoma Cell Line.Cancers(Basel),2019,12(1):61.)

Method used

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  • Biomarker related to oral squamous carcinoma
  • Biomarker related to oral squamous carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 QPCR detection of expression levels of biomarkers

[0052] 1. Sample collection

[0053] Fifteen oral squamous cell carcinoma tissues and 15 corresponding paracancerous tissues were collected. The patients did not receive any treatment before operation. The cancer tissues were used as the experimental group, and the paracancerous tissues were used as the normal control group.

[0054] 2. RNA extraction

[0055] The RNA in the sample was extracted using QIAGEN tissue RNA extraction kit, and the detailed operation was carried out according to the instructions.

[0056] 3. QPCR detection

[0057] 1) Use the FastQμant cDNA First Strand Synthesis Kit (Product No.: KR106) for mRNA reverse transcription;

[0058] 2) Design QPCR amplification primers based on the sequences of RP13-297E16.4 and C20orf197, synthesized by Biomed Biotech, using GAPDH as an internal reference gene.

[0059] Use SuperReal PreMix Plus (SYBR Green) (product number: FP205) to amplify, and ...

Embodiment 2

[0062] Example 2 Diagnostic efficacy verification of biomarkers

[0063] The preprocessed oral squamous cell carcinoma sequencing data and clinical information were downloaded from the TCGA database, and the sample size was paracancerous:carcinoma=44:331.

[0064] Using R software DESeq2 to analyze differential lncRNA expression, the results showed that RP13-297E16.4 and C20orf197 were significantly upregulated in OSCC tissues.

[0065] The R package "pROC" was used to draw the receiver operating curve (ROC), analyze the AUC value, sensitivity and specificity, and judge the diagnostic efficacy of indicators alone or in combination. When judging the diagnostic efficacy of the combination of indicators, perform logistic regression on the expression level of each gene, calculate the probability of each individual suffering from cancer or not through the fitted regression curve, determine different probability division thresholds, and divide according to the determined probability...

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Abstract

The invention discloses a biomarker related to oral squamous carcinoma. The biomarker is selected from RP 13-297E16.4 and / or C20orf197. Experiments prove that RP13-297E16.4 and C20orf197 are remarkably up-regulated in oral squamous carcinoma and have a relatively high AUC value, which indicates that RP13-297E16.4 and / or C20orf197 have relatively high accuracy when applied to diagnosis of oral squamous carcinoma.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a biomarker related to oral squamous cell carcinoma, specifically RP13-297E16.4 and / or C20orf197. Background technique [0002] Oral squamous cell carcinoma (OSCC) is an important branch of head and neck squamous cell carcinoma (HNSCC), accounting for more than 90% of oral malignancies, and is the sixth most common malignancy in the world (Silva LC, Fonseca FP, Almeida OP, et al.CD 1a+and CD207+cells are reduced inoral submucous fibrosis and oral squamous cell carcinoma.Med Oral Patol OralCir Buca1,2020,25(1):49-55.), second only For lung cancer, gastric cancer, breast cancer, colorectal cancer and cervical cancer (JohnsonN. Tobacco use and oral cancer: A global perspective. J. Dent. Educ 2001, 65: 328-339.). About 1 / 3 of OSCC originates from potential precancerous lesions of the oral cavity such as oral leukoplakia (OL) and oral submucosal fibrosis (OSMF). OSCC has the characteristics o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K45/00A61P35/00
CPCC12Q1/6886A61K45/00A61P35/00C12Q2600/158C12Q2600/136
Inventor 王月红王弦唐瞻贵陈晓婧陈佩雯沈永祥刘海李步云李熠洁严思
Owner XIANGYA STOMATOLOGICAL HOSPITAL CENT SOUTH UNIV
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