SSR (simple sequence repeat) marker fingerprint spectrum of hypsizigus marmoreus Finc-B-6 strain as well as construction method and application of SSR marker fingerprint spectrum

Abstract

The invention discloses an SSR (simple sequence repeat) marker fingerprint spectrum of a hypsizigus marmoreus Finc-B-6 strain as well as a construction method and application of the SSR marker fingerprint spectrum. The SSR marker fingerprint spectrum consists of 6 pairs of SSR markers. The construction method comprises the following steps: (1) conducting mycelium culture; (2) extracting genome DNA (deoxyribose nucleic acid); (3) detecting the SSR molecular markers; and (4) conducting capillary electrophoresis detection. The application comprises the following steps: the hypsizigus marmoreus strain is subjected to SSR marker amplification, the obtained banding pattern is compared with the fingerprint spectrum, and if the banding pattern is consistent with the fingerprint spectrum, the strain is determined to be the hypsizigus marmoreus Finc-B-6 strain. Compared with the conventional morphological detection, antagonism test and fruiting test, the method has the advantages of short detection time, high accuracy and good repeatability, and has the specificity of the hypsizigus marmoreus Finc-B-6 strain in 56 collected hypsizigus marmoreus main culture strains at home and abroad.

Description

technical field [0001] The invention belongs to the technical field of detection of mushroom strains, and in particular relates to an SSR marker fingerprint of mushroom strain Finc-B-6 and its construction method and application. Background technique [0002] True Ji mushroom (Latin name Hypsizygus marmoreus (Peck) H.E.Bigelow) belongs to Basidiomycota, Agaricaceae, Agaricaceae, Liphyllaceae, and is named for the marble pattern on the surface of its cap. Its beautiful appearance, crisp and tender texture, unique seafood flavor and economical price are loved by consumers. Shiji mushroom is not only rich in vitamins and 18 kinds of amino acids, recent studies have also found that small molecular compounds, polysaccharides and peptides contained in Shiji mushroom have hemolysis, anti-oxidation, inhibition of leukemia and lymphoma cells, lowering blood lipids, and anti-inflammation and anti-oxidation functions, it is a kind of food and medicine with high economic value. Since ...

AI Technical Summary

Problems solved by technology

Although the output of factory production has increased year by year, some strains still have problems such as long cultivation period, low yield per unit area, poor appearance uniformity of single root fruiting bodies, and easy occurrence of wart caps. These defects may lead to increased production costs and reduced product quality. Market Competitiveness

On the other hand, due to the low diversity of the strains produced in the industrial production of jiji mushrooms, the appearance of the products is similar, which cannot meet the diverse needs of market consumers.

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