Fluorescence excitation system, optical system and real-time fluorescence quantitative PCR instrument

Abstract

The invention discloses a fluorescence excitation system, an optical system and a real-time fluorescence quantitative PCR instrument. The fluorescence excitation system comprises a first free-form surface reflector, at least two excitation light sources and a sample table, wherein the at least two excitation light sources are positioned between the first free-form surface reflector and the sample table; and excitation light emitted by the at least two excitation light sources is reflected by the first free-form surface reflector and is converged on the sample table. Multiple paths of independent excitation light sources are adopted, and through reflection of the free-form surface reflector, all the paths of excitation light are converged on the sample table and used for exciting fluorescence. When the spectral energy of a certain wave band needs to be improved, only the intensity of the excitation light source corresponding to the spectrum of the wave band needs to be improved; and if one or more of the multiple excitation light sources are damaged, the damaged excitation light source can be directly replaced, and the use of the whole real-time fluorescent quantitative PCR instrument is not influenced.

Description

technical field [0001] The present application relates to the technical field of optical systems, in particular to a fluorescence excitation system, an optical system and a real-time fluorescence quantitative PCR instrument. Background technique [0002] Real-time fluorescence quantitative PCR (Quantitative Real-time PCR) is a method for detecting the total amount of products after each polymerase chain reaction (PCR) cycle with fluorescent chemicals in DNA amplification reactions. Real-time fluorescent quantitative PCR instrument is an instrument that detects the fluorescence signal intensity of PCR samples through an optical system, thereby quantitatively analyzing specific DNA sequences in PCR samples, for example, for nucleic acid detection. The optical system of the real-time fluorescence quantitative PCR instrument mainly includes a fluorescence excitation system for generating excitation light and a fluorescence detection system for receiving fluorescence. [0003] D...

AI Technical Summary

Problems solved by technology

[0006] In order to solve the existing technology, when it is necessary to increase the spectral energy of a certain band, it can only be achieved by increasing the light intensity of the overall continuous light source, which will be accompanied by a lot of energy waste and generate a lot of heat; in addition , if the filter switching mechanism fails due to aging or is damaged during transportation, the entire real-time fluorescent quantitative PCR instrument will not work properly

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