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Kit for detecting fat-soluble vitamins and detection method

A technology of fat-soluble vitamins and kits, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve the problems of simultaneous detection of multiple fat-soluble vitamins

Pending Publication Date: 2021-05-25
合肥和合医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, a single fat-soluble vitamin can be detected by high performance liquid chromatography, but there is no method for simultaneous detection of multiple fat-soluble vitamins

Method used

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  • Kit for detecting fat-soluble vitamins and detection method
  • Kit for detecting fat-soluble vitamins and detection method
  • Kit for detecting fat-soluble vitamins and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] Embodiment 1: The kit for detecting fat-soluble vitamin

[0115] The main components of the kit are shown in Table 1:

[0116] Table 1

[0117] Composition Component identification dosage form Specification Fat Soluble Vitamin Calibrator C1, C2, C3, C4, C5, C6 solid 6 bottles Fat-Soluble Vitamin Controls QC(L), QC(M), QC(H) solid 3 bottles Mixed internal standard protein precipitant P14 liquid 24mL×1 bottle Mobile Phase Additive A A10 liquid 8mL×1 bottle Flow Additive B B11 liquid 5mL×1 bottle extract T12 liquid 24mL×1 bottle Reconstitution solution F13 liquid 12mL×1 bottle

[0118] Specifically, after the fat-soluble vitamin calibrator and the fat-soluble vitamin quality control were reconstituted in 1 mL of water, the concentration of each fat-soluble vitamin was as follows:

[0119] Fat-soluble vitamin calibrator C1: vitamin A is 0.056±0.011μg / mL, vitamin E is 1.125±0.23μg / mL...

Embodiment 2

[0136] Embodiment 2: the detection method of fat-soluble vitamin

[0137] The embodiment of the present invention provides a method for detecting fat-soluble vitamins based on the kit in Example 1 above, such as figure 2 shown, including:

[0138] Step 201: adding aqueous solutions to at least three bottles of fat-soluble vitamin calibration products in the kit to redissolve to obtain standard working solutions with at least three concentrations;

[0139] Step 202: Add the mixed internal standard protein precipitation agent in the kit to the standard working solution of at least three concentrations respectively to obtain standard solutions of at least three concentrations, wherein the mixed internal standard protein in the standard solution of at least three concentrations The same amount of precipitant;

[0140] Step 203: using the mobile phase additive in the kit to prepare the mobile phase;

[0141] Step 204: Using liquid chromatography-mass spectrometer and mobile pha...

Embodiment 3

[0150] Embodiment 3: prepare the standard solution of series concentration

[0151] Add 1 mL of aqueous solution to the six bottles of fat-soluble vitamin calibration products in Example 1 to redissolve to obtain six different concentrations of standard working solutions, and use a pipette to pipette 200 μL of six different concentrations of standard working solutions. 210 μL of the mixed internal standard protein precipitant in the 96-well plate were placed in each well of the 96-well plate, mixed to make six kinds of mixed solutions with different concentrations, and the amount of the internal standard in the six standard solutions was the same, and then added to the above mixed solution Add the extractant and the extract in the kit, vortex mix and centrifuge, and the centrifuged upper layer solution is the standard solution.

[0152] It should be noted that the standard solutions with different concentrations can be obtained according to the pretreatment operation when proc...

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Abstract

The invention provides a kit for detecting fat-soluble vitamins and a detection method. The kit comprises at least three bottles of fat-soluble vitamin calibration products, at least three bottles of fat-soluble vitamin quality control products, a mixed internal standard protein precipitator, a mobile phase additive and an extracting solution, the at least three bottles of fat-soluble vitamin calibration products and the at least three bottles of fat-soluble vitamin quality control products are freeze-dried products of fat-soluble vitamins, and the fat-soluble vitamins comprise vitamin A, vitamin E, 25-hydroxy vitamin D2, 25-hydroxy vitamin D3, vitamin K1 and vitamin K2; the mixed internal standard protein precipitant comprises a mixed solution containing a protein precipitant, a vitamin A-d3 internal standard substance, a vitamin E-d6 internal standard substance, a 25-hydroxy vitamin D2-d3 internal standard substance, a 25-hydroxy vitamin D3-d6 internal standard substance, a vitamin K1-d7 internal standard substance and a vitamin K2-d7 internal standard substance; and the mobile phase additive comprises a methanol solution containing formic acid and an acetonitrile solution containing formic acid. The method can be used for detecting various fat-soluble vitamins at the same time.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a kit and a detection method for detecting fat-soluble vitamins. Background technique [0002] Fat-soluble vitamins are naturally occurring micronutrients in foods that provide and maintain essential and broad physiological and biochemical functions. Fat-soluble vitamins are a class of vitamins that are soluble in organic solvents but not in water, including vitamin A, vitamin E, 25-hydroxyvitamin D2, 25-hydroxyvitamin D3, vitamin K1 and vitamin K2. [0003] Vitamin A is the prosthetic group of enzymes, regulates the metabolism of organisms, and is one of the indispensable and important nutrients for growth and development. Vitamin E is the general term for tocopherol and tocotrienol, which participates in the metabolism of fat and maintains the normal function of endocrine. Vitamin D is a steroid derivative, and its active forms are 25-hydroxyvitamin D2 and 25-hyd...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72G01N2030/045
Inventor 韦弢倪君君高霞刘飞
Owner 合肥和合医疗科技有限公司
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