Application of elaeocarpus nitentifolius extract in preparation of medicines for treating stomach-related diseases
A technology of extracts and medicines, applied in the field of medicine, can solve problems such as the lack of reports on the research on the treatment of stomach diseases by Du Ying with bright leaves
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Embodiment 1
[0017] The preparation (water extraction extract) of embodiment 1 bright leaf Du Ying extract
[0018] 1 kg of the branches and leaves of Du Ying with distilled water were extracted three times under hot reflux with distilled water, each time for one hour, and the extract was concentrated under reduced pressure to obtain 70 g of water extract.
Embodiment 2
[0019] The preparation of embodiment 2 Du Ying radix extract (example of alcohol extraction extract)
[0020] 2.2 kg of Du Ying leaves, add 6 times the amount of 90% ethanol, soak overnight or 4 hours, slowly heat to boiling, reflux for 1 hour, filter; repeat the whole process twice, combine the filtrate, concentrate under reduced pressure, temperature 60-70 ℃, Pressure 85-98KPa, when concentrated to completely free of ethanol, add water of the same weight as the medicinal materials and continue heating until boiling, after cooling, centrifuge to remove insoluble matter, and add a small amount of water to wash the insoluble matter three times, combine the filtrates, and reduce pressure at a temperature of 60-70C Concentrate, then dry under reduced pressure (temperature 70-80°C, pressure 85-98Kpa) to obtain 147g of extract.
[0021] Pharmacological experiment:
[0022] The samples used in the pharmacological experiments are the samples obtained from the preparation of the extr...
experiment example 1
[0023] Experimental example 1. the extract of Duying radix L. to H + -K + -ATPase activity effect
[0024] Method: take a 96-well plate, add lysozyme, 1.0mg / ml and 0.25mg / ml of the extract of Duying radix, rabeprazole sodium 10 -3 mol / L and 10 -4 mol / L, the volume is 20ul. Add pig H diluted 20 times with the reaction solution + -K + -ATPase 160ul, incubate at 37°C for 20 minutes, add 20mg / ml ATP 20ul, blank without adding ATP. React at 37°C for 30 minutes. Add 50ul of 10% TCA to terminate the reaction. Blank plus 20mg / ml ATP20ul. Take 50 μl and place it in a 96-well microtiter plate, add 200ul of phosphorous solution for color reaction, and measure the color at 660nm. Calculate the inhibition rate, inhibition rate=(control-sample) / (control-blank)×100%.
[0025] RESULTS: The extract of Duying radix had a positive effect on pig stomach H + -K + -ATPase activity has a strong inhibitory effect, and the inhibition rates of 100ug / ml and 20ug / ml are 58% and 26%, respectiv...
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