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A kind of high-strength silk protein nanofiber membrane and its preparation method

A nanofiber membrane and nanofiber technology, which is applied in the field of high-strength silk protein nanofiber membrane and its preparation, can solve the problems of measurement and regenerated silk nanofiber membrane fragility and fragility, and achieve the improvement of tensile mechanical properties and simple preparation method The effect of easy operation and high tensile mechanical strength

Active Publication Date: 2022-05-17
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, due to the high charge density of silk protein nanofibers, the formation of effective non-covalent interactions between nanofibers is inhibited, and the formed regenerated silk nanofiber membranes are fragile and fragile, and cannot even be measured by traditional tensile models.

Method used

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  • A kind of high-strength silk protein nanofiber membrane and its preparation method
  • A kind of high-strength silk protein nanofiber membrane and its preparation method
  • A kind of high-strength silk protein nanofiber membrane and its preparation method

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preparation example Construction

[0027] The invention provides a method for preparing a high-strength silk protein nanofiber membrane, comprising the following steps:

[0028] S1: Freeze-dry the aqueous solution of high-crystalline fibroin nanofibers to obtain freeze-dried powder of silk protein nanofibers; the crystallinity of silk protein fibers in the aqueous solution of high-crystalline fibroin nanofibers is ≥ 40%, the diameter is 10-30 nm, and the length is 200-200 nm. 2000nm;

[0029] S2: Dissolving the lyophilized powder of silk protein nanofibers in a formic acid solution to obtain a formic acid solution of silk protein nanofibers;

[0030] S3: volatilize formic acid in the formic acid solution of the silk protein nanofibers to form a film to obtain a silk protein nanofiber film.

[0031] The high crystal fibroin nanofibers used in the method provided by the invention have a beta-sheet crystal structure and good stability, and can still maintain its original secondary structure and nanofiber morpholo...

Embodiment 1

[0047] (1) freeze-drying 0.5% silk protein nanofiber aqueous solution to obtain silk protein nanofiber freeze-dried powder;

[0048] (2) Dissolving the lyophilized powder of silk protein nanofibers in formic acid solution at 60° C. for 0.5 h to obtain a 10% formic acid solution of silk protein nanofibers;

[0049] (3) A 10% formic acid solution of silk protein nanofibers was volatilized at 60° C. for 2 hours to prepare a silk protein nanofiber film with a thickness of 30 μm.

[0050] figure 1 Atomic force microscope pictures of silk protein nanofibers prepared in Example 1 of the present invention dissolved in water (a) and formic acid (b) respectively; from figure 1 It can be seen that: in the aqueous solution, the silk fibroin in the formic acid solution all presents the shape of nanofibers; the length of the nanofibers in the aqueous solution is distributed in 200-1000nm, while the length of the nanofibers in the formic acid is reduced to 50-200nm, and the shorter fibers ...

Embodiment 2

[0059] (1) freeze-drying 2% silk protein nanofiber aqueous solution to obtain silk protein nanofiber freeze-dried powder;

[0060] (2) Dissolving the lyophilized powder of silk protein nanofibers in formic acid solution at 20° C. for 1 hour to obtain a 2% formic acid solution of silk protein nanofibers;

[0061] (3) A 2% silk protein nanofiber formic acid solution was volatilized at 20° C. for 72 hours to prepare a silk protein nanofiber film with a thickness of 38 μm.

[0062] The tensile mechanical strength of the silk protein nanofiber membrane prepared in Example 2 is 69.2 MPa and 13.5 MPa in dry state and wet state, respectively.

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Abstract

The invention provides a high-strength silk egg nanofiber film and a preparation method thereof. The aqueous solution of high-crystalline silk protein nanofibers is freeze-dried to obtain silk protein nanofiber freeze-dried powder; dissolved in formic acid solution to obtain silk protein nanofiber formic acid The solution is volatilized to form a film to obtain a silk protein nanofiber film. The high-crystalline fibroin nanofibers used in this method have a beta-sheet crystal structure and good stability, and can still maintain their original secondary structure and nanofiber morphology when dissolved in formic acid, and due to the optimization of non-covalent interactions The tensile mechanical properties of the prepared membrane material are greatly improved. The invention realizes the transition of mechanical properties from fragile to strong and tough through simple solvent conversion, and shows high tensile mechanical strength in dry state and wet state, reaching 69.2-76.9MPa and 13.5-14.6MPa respectively, and in It is carried out under normal temperature and pressure, and the preparation method is simple and feasible.

Description

technical field [0001] The invention belongs to the technical field of high-strength biological materials, and in particular relates to a high-strength silk protein nanofiber membrane and a preparation method thereof. Background technique [0002] Due to its excellent performance and wide application, high-strength materials have always been a research hotspot in the field of materials. Natural high-strength materials self-assemble into complex micro-nano structures by optimizing the non-covalent interactions between components, giving them excellent properties. For example, cellulose in plants, chitin in arthropod bones, and silk protein in animal silk all form nanofibers of different sizes and are formed by optimizing non-covalent interactions such as hydrogen bonds, electrostatics, and hydrophobic interactions. Functional materials with high strength, stiffness and toughness. Therefore, regulating the supramolecular assembly of nanofibrous units at the micro-nano scale ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J5/18C08L89/00
CPCC08J5/18C08J2389/00
Inventor 吕强张筱旖
Owner SUZHOU UNIV
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