Preparation method and application of human amniotic stem cell culture

Abstract

The invention relates to the technical field of biomedicine, and discloses a preparation method of a human amniotic stem cell culture. The preparation method comprises the following steps of: firstly, culturing separated and extracted human amniotic stem cells by using a human amniotic stem cell culture medium; and when the human amniotic stem cells are amplified to a cell confluence degree of 90%, replacing a serum-free culture medium to continuously culture the human amniotic stem cells for 48 hours; then collecting supernatant (namely the culture), and concentrating the supernatant by 10 times to obtain the human amniotic stem cell culture. The invention also discloses application. The invention discovers and proves that the human amniotic stem cell culture has the effects of whitening skin, brightening skin color and keeping the skin healthy and natural for the first time, has good prevention and treatment effects on melanin deposition caused by endocrine dyscrasia or chemical substance stimulation or physical method stimulation, is safe in components, does not have immunological rejection or tumorigenic effect, and does not have obvious toxic or side effect on a human body; the preparation method is simple, the operation process is controllable, and the preparation method has important industrialization value.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method and application of human amniotic membrane stem cell culture. Background technique [0002] Melanin deposition is the excessive deposition of abnormally increased melanin in certain parts of the skin, and then forms pigment spots, age spots, etc. Ultraviolet radiation, endocrine disorders, and bad living habits can lead to skin elasticity loss, pigmentation, and overall darker and darker skin, which seriously affects the appearance of human skin, especially under the influence of "white is beautiful" Asian culture, fair skin in Aesthetics occupies a very important position. Therefore, the global whitening skin care market is huge. Among the functional skin care products, the proportion of whitening and lightening spots is the highest. Many multinational companies only sell whitening products, and their annual revenue can reach hundreds of millions of dol...

AI Technical Summary

Problems solved by technology

However, these compounds all have the following obvious disadvantages: kojic acid has sensitization and potential carcinogenic risks, arbutin is a hydroquinone derivative, and hydroquinone is oxidized into toxic semiquinone under the action of tyrosinase Substances that can cause skin erythema, desquamation, itching, stinging, allergies, and even permanent skin damage, and are carcinogenic; vitamin C derivatives are only effective at high concentrations, and it is difficult to stabilize them in cosmetic formulations present; alpha-hydroxy acids are very irritant

In comparison, there is currently no preparation product that promotes the degradation of melanin

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