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Metabolic Enzyme Induced Biodegradation of Carbon Black Granules and Analytical Method of Its Products

A technology of biodegradation and degradation products, applied in the field of analysis of biodegradation and its products, it can solve the problems of being difficult to be biodegraded, and the chemical properties of carbon black are inert, so as to promote desorption and ionization, reduce the co-crystallization process, and achieve high sensitivity. Effect

Active Publication Date: 2022-02-18
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the chemical properties of carbon black are relatively inert and difficult to be biodegraded.

Method used

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  • Metabolic Enzyme Induced Biodegradation of Carbon Black Granules and Analytical Method of Its Products
  • Metabolic Enzyme Induced Biodegradation of Carbon Black Granules and Analytical Method of Its Products
  • Metabolic Enzyme Induced Biodegradation of Carbon Black Granules and Analytical Method of Its Products

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Embodiment

[0072] Prepare an aqueous dispersion of carbon black particles at a certain concentration, mix the carbon black particles with liver metabolic enzymes (cytochrome P450, glutathione transferase), and then put them into a water-proof incubator for incubation. The mixture was cultured continuously for 14 days. To simulate the biological environment, these experiments were first performed in a dark environment at 37 °C. After finding that carbon black particles can be degraded by metabolic enzymes, the applicant also carried out light irradiation experiments to test the application potential of this technology in wastewater purification. The light intensity is maintained at 550W / m 2 , the solution is monitored at certain time intervals to monitor the conversion of carbon black particles. All experiments were repeated three times (n=3). This application uses the fly ash sample as an example of an actual sample here. The pretreatment process of the actual sample is as follows: f...

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Abstract

A method for analyzing the biodegradation of carbon black particles induced by metabolic enzymes and its products, comprising: step (1), taking an appropriate amount of carbon black samples; step (2), dispersing the weighed carbon black samples with water and preparing certain concentration of the dispersion; step (3), the carbon black dispersion is mixed with the metabolic enzyme solution, and the mixed solution is placed in a vortex shaker to mix; step (4), the mixed solution is placed in a water-proof incubator for incubation . The method of the present invention reveals the action of the enzyme through complementary multiplex techniques, demonstrating that it can induce the transformation of carbon black particles. According to the typical mass spectrum peaks of carbon clusters and the changes of carbon-oxygen and carbon-nitrogen bonds in the spectrum, the transformation mechanism of carbon black particles in organisms was proposed.

Description

technical field [0001] The invention belongs to the field of biodegradation and its analysis, and more specifically relates to an analysis method based on the biodegradation of carbon black particles induced by metabolic enzymes and its products. Background technique [0002] Carbon black particles are the products of incomplete combustion or thermal decomposition of hydrocarbons. Because of their low cost, high conductivity, and small size, they are widely used as reinforcing fillers for filters, elastomers, plastics, paints, and tires, fuel cells and Conductive additives and catalyst supports in electrode materials. The large amount of these particles emitted into the environment also increases the risk of human exposure. A large number of epidemiological results show that carbon black has extremely strong toxicity and biological effects, and has a great impact on human health. Its toxicity is much higher than other fine particles. Carbon black has small particle size, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/623G01N27/624G01N1/38
CPCG01N27/622G01N1/38
Inventor 刘倩黄秀江桂斌
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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