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EGCG-CoPt NPs-Apt nanoparticles as well as preparation method and application thereof

A nanoparticle, SH-AS1411 technology, applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., to achieve the effects of reducing membrane penetration, inhibiting tumor cell metastasis, and reducing proliferation rate

Pending Publication Date: 2021-06-11
福州市第二医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, so far, the technology of using EGCG as a template to prepare nanomaterials and couple nucleic acid aptamers to inhibit tumor cells has not been reported.

Method used

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  • EGCG-CoPt NPs-Apt nanoparticles as well as preparation method and application thereof
  • EGCG-CoPt NPs-Apt nanoparticles as well as preparation method and application thereof
  • EGCG-CoPt NPs-Apt nanoparticles as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment E

[0024] Preparation of Example EGCG-CoPt NPs Nanoparticles

[0025] Step (1) prepares EGCG-CoPt NPs nanoparticles:

[0026] 5 mL of 1M CoCl 2 solution with 10mL 0.5M K 2 PtCl 4 The solutions were mixed to obtain a mixed solution. After shaking and shaking in a water bath at 25°C for 30 minutes, 0.125 g of EGCG was added, and the water bath was continued to shake and shake for 30 minutes; then, 1 mL of 0.6M sodium borohydride solution was added under ultrasound to react for 30 minutes, centrifuged, and removed. Washing with deionized water for 3 times and suspending with deionized water to obtain EGCG-CoPt NPs nanoparticle suspension.

[0027]Step (2) prepares EGCG-CoPt NPs-Apt nanoparticles:

[0028] Add 1 L of thiol nucleic acid aptamer SH-AS1411 to the EGCG-CoPt NPs nanoparticle suspension prepared in step (1), and incubate overnight at 4°C to obtain the EGCG-CoPt NPs-Apt nanoparticle suspension.

[0029] The EGCG-CoPt NPs-Apt nanoparticles prepared by the present invent...

experiment example 1

[0033] Experimental example 1 Study on the inhibitory effect of EGCG-CoPt NPs-Apt nanoparticles on the proliferation of triple-negative breast cancer cells MDA-MB-231

[0034] (1) Experimental steps:

[0035] The experimental group was divided into three groups, namely CoPt NPs group, EGCG-CoPt NPs group and EGCG-CoPt NPs-Apt group. Take the MDA-MB-231 cells cultured to the logarithmic growth phase, and adjust the cell concentration to 5×10 5 cells / mL, take 100 μL cell suspension and inoculate into 96-well culture plate, place at 37°C, volume fraction 5% CO 2 After incubating in the incubator for 6 h, the fresh medium was replaced. According to different groups, 50 μL of CoPt NPs nanoparticle suspension, 50 μL of EGCG-CoPtNPs-Apt nanoparticle suspension, and 50 μL of EGCG-CoPt NPs nanoparticle suspension were added to the experimental group, and 50 μL of fresh medium was added to the blank control group. Set up 3 parallels. Continue to place at 37°C, 5% volume fraction CO ...

experiment example 2

[0041] Experimental example 2 Study on the inhibitory effect of EGCG-CoPt NPs-Apt nanoparticles on the migration of triple-negative breast cancer cells MDA-MB-231

[0042] Take the MDA-MB-231 cells cultured to the logarithmic growth phase, and adjust the cell concentration to 5×10 5 individual / mL. Add 500 μL of RPMI 1640 medium containing 10% fetal bovine serum to the lower chamber of the Transwell chamber, and add 100 μL of cell suspension to the upper chamber. The experimental group was added 50 μL CoPt NPs nanoparticle suspension, 50 μL EGCG-CoPtNPs-Apt nanoparticle suspension, 50 μL EGCG-CoPt NPs nanoparticle suspension according to the different groups, and the control group was added 50 μL fresh medium. Next, place at 37°C, 5% volume fraction CO 2 Cultivate in an incubator for 24 hours, discard the medium, fix with absolute ethanol, and stain with 1 g / L crystal violet for 10 minutes each. Five fields of view were randomly selected under a magnification of 400 times, a...

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Abstract

The invention belongs to the field of biological medicine, and relates to an EGCG-CoPtNPs-Apt nanoparticle as well as a preparation method and application thereof. According to the EGCG-CoPt NPs-Apt nanoparticles as well as the preparation method and application thereof, EGCG is taken as a template, EGCG-CoPtNPs nanoparticles are synthesized in a green manner, and then the surfaces of the EGCG-CoPtNPs nanoparticles are modified with specific nucleic acid aptamers AS1411 of triple negative breast cancer cells, so that the composite EGCG-CoPtNPs-Apt nanoparticles with a molecular recognition function are formed. According to the EGCG-CoPtNPs-Apt nanoparticles prepared by the invention, the dual targeting effect of the nanoparticles and the nucleic acid aptamers, the proliferation rate and the transmembrane effect of the triple negative breast cancer cells MDA-MB-231 can be remarkably reduced, meanwhile, the expression quantity of tumor cell transfer factors MMP-2, MMP-9, EGFR and VASP is reduced, tumor cell metastasis is inhibited, and a foundation is laid for clinical triple-negative breast cancer diagnosis and treatment integration.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an EGCG-CoPt NPs-Apt nano particle and a preparation method and application thereof. Background technique [0002] In recent years, due to changes in various social factors, the incidence of breast cancer has shown an upward trend year by year, and has become a serious threat to the health of women in my country. Its recurrence, metastasis and distant metastasis are characterized by high mortality. the main reason. Triple-negative breast cancer (TNBC) refers to a subtype of breast cancer that does not express estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). Because of its strong invasiveness and high degree of malignancy, and patients with this type of breast cancer cannot benefit from endocrine therapy and anti-HER2 targeted therapy, systemic chemotherapy is mainly used clinically, but the curative effect obtained in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/54A61K47/69A61K31/353A61K31/7088A61K33/243A61P35/00A61P35/04
CPCA61K31/353A61K31/7088A61K47/549A61K47/6929A61P35/00A61P35/04A61K33/243A61K2300/00
Inventor 陈国平
Owner 福州市第二医院
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