Rice antihypertensive peptide and enzymolysis preparation method thereof

Abstract

The invention discloses a rice antihypertensive peptide and an enzymolysis preparation method thereof, the amino acid sequence of the rice antihypertensive peptide is ACHHQENYGNPTV, and the enzymolysis preparation method of the rice antihypertensive peptide comprises the following steps: (1) pretreatment; (2) grinding treatment; (3) performing enzymolysis treatment; (4) centrifugation and ultrafiltration treatment; (5) adsorption treatment; and (6) vacuum freeze drying treatment. The antihypertensive peptide prepared by the invention has good ACE (angiotensin converting enzyme) inhibitory activity, and the IC50 value of the antihypertensive peptide is less than 0.069 mg / mL; meanwhile, the antioxidant activity of the antihypertensive peptide is very good, and the scavenging rate of DPPH free radicals is as high as 91.5%. An in-vivo model test shows that the antihypertensive peptide has better antihypertensive activity, has a treatment effect close to that of a medicine captopril, and can be used as a potential oral liquid product; meanwhile, after the antihypertensive peptide is taken for 24 hours, only slight blood pressure up-regulation occurs in a group using the antihypertensive peptide, which shows that the antihypertensive peptide prepared by the invention has a slow-release antihypertensive effect.

Description

technical field [0001] The invention relates to the technical field of biomanufacturing, in particular to a rice hypotensive peptide and an enzymolysis preparation method thereof. Background technique [0002] Bioactive peptides refer to peptide compounds that are beneficial to the life activities of living organisms or have physiological effects, also known as functional peptides, whose physiological functions mainly include antibacterial activity, immune activity, antioxidant effect, antihypertensive activity, cholesterol-lowering effect Combine minerals, promote growth, antithrombotic effect, inhibit tumor metastasis, etc. [0003] At present, the earliest countries in the world to study rice and rice bran resources are the United States and Japan. American Rice Innovation Company and Lippman Company are far ahead of other companies in this respect. They have developed various rice bran nutritional foods with clear functional factors and exact health effects, such as sol...

AI Technical Summary

Problems solved by technology

At present, the separation and purification technology of rice ACE inhibitory peptide is general, and the activity after purification is low. At the same time, there are few reports on its animal experiments, especially in vivo gavage experiments.

Images