Method for preparing active peptide of laver

Abstract

The invention discloses a method for preparing active peptide of laver. The method is characterized by comprising the following steps of: drying and crushing porphyra yezoensis serving as a raw material, taking the part with particle size of between 150 and 200 meshes, adding water into the raw material according to a weight volume ratio of the raw material to water as 1 to 100g / mL and uniformly mixing; filling the material liquid into an ultrasonic cell disruptor for disruption and centrifuging to obtain supernatant; adjusting the pH value of the supernatant to be 7.0, adding papain for enzymolysis, inactivating, cooling and centrifuging to obtain enzymatic hydrolyzate; and performing ultrafiltration on the enzymatic hydrolyzate, concentrating filtrate and performing vacuum freeze drying to obtain a freeze-dried product of the active peptide of the laver. The method has the advantages of more reasonable preparation process, simple process, high operability and high extraction rate; the prepared active peptide of the laver contains fewer impurities; and the biological activity is also kept to the greatest extent.

Description

technical field

[0001] The invention relates to a preparation method of an active peptide, in particular to a preparation method of a laver active peptide. Background technique

[0002] The Chinese published patent document with the publication number CN1687444 and the application number CN200510038532.5 discloses a "method for preparing laver hypotensive peptide using enzyme-membrane coupling technology and its application", which is the preparation of laver antihypertensive peptide by enzyme-membrane coupling technology. In the method of the blood pressure peptide, the proteases selected are trypsin, compound protease, pepsin, alkaline protease, neutral protease and the compound enzymes composed of them. The core technology of this invention is the enzyme-membrane coupling technology, and the product yield is improved, but it also has the disadvantages of complicated operation and difficult realization in actual production, which is different from the papain used in the p...