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A detection reagent and detection kit for detecting imatinib and/or n-desmethyl imatinib

A technology of methyl imatinib and detection reagents, which is applied in the field of immunological detection, can solve the problems of unrecognizable specificity and lack of high sensitivity, and achieve simple operation and strong specificity

Active Publication Date: 2022-08-09
BEIJING DIAGREAT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent document does not specify whether to recognize N-desmethyl imatinib, but it cannot be recognized from the description of specificity
[0007] In summary, there is currently a lack of immunological detection methods with high sensitivity and simultaneous determination of imatinib and N-desmethylimatinib

Method used

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  • A detection reagent and detection kit for detecting imatinib and/or n-desmethyl imatinib
  • A detection reagent and detection kit for detecting imatinib and/or n-desmethyl imatinib
  • A detection reagent and detection kit for detecting imatinib and/or n-desmethyl imatinib

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Experimental program
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Embodiment Construction

[0067] The present application will be further described in detail below with reference to the examples.

[0068] Preparation example of haptens of imatinib and N-desmethyl imatinib

[0069] The structures of the haptens of imatinib and N-desmethyl imatinib are shown in formula 10, and the specific synthetic route of the compound of formula 10 is as follows:

[0070]

[0071] Using carboxybenzaldehyde and trimethyl orthoformate as starting materials, the target product imatinib is obtained through seven steps such as aldehyde group protection reaction, condensation reaction, reductive amination reaction, substitution reaction, oxidation reaction and hydrolysis reaction. and N-desmethyl imatinib hapten.

[0072] Step (i): the compound shown in formula 1 and trimethyl orthoformate undergo aldehyde group protection reaction to obtain the compound shown in formula 2:

[0073] To a 500mL single-necked flask, add p-carboxybenzaldehyde (15g, 0.01mol), 150mL methanol, p-toluene...

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Abstract

The application relates to the technical field of immunological detection, and specifically discloses a detection reagent for detecting imatinib and / or N-desmethyl imatinib, including an enzyme-labeled working solution, a magnetic particle working solution, an indicator reagent solution; enzyme labeling working solution includes conjugates obtained from haptens and markers; magnetic particle working solution includes conjugates obtained by coupling antibodies and magnetic particles; indicator solution includes a reagent for quantitative detection of marker concentration Indicator; the structure of a hapten is shown in formula 10: an antibody is generated in response to an antigen, and the antigen is a conjugate formed by coupling a hapten and a carrier protein. This application can simultaneously measure imatinib and N-desmethyl imatinib, with strong specificity and sensitivity.

Description

technical field [0001] The present application relates to the technical field of immunological detection, and more particularly, to a detection reagent and a detection kit for detecting imatinib and / or N-desmethyl imatinib. Background technique [0002] Imatinib mesylate is a small molecule tyrosine kinase inhibitor that targets KIT and platelet-derived growth factor receptor (PDGFRA) to exert anti-tumor activity, mainly for the treatment of Philadelphia chromosome (Bcr-Abl) positive chronic myeloid leukemia (CML) and unresectable or metastatic malignant gastrointestinal stromal tumor (GIST) patients. [0003] Imatinib is mainly metabolized into N-desmethylpiperazine derivative (N-desmethylimatinib) by CYP3A enzyme system in the human body, and the in vitro efficacy of this metabolite is similar to that of the original drug. There is therefore a need for assays capable of measuring imatinib and N-desmethyl imatinib simultaneously. In recent years, studies have shown that t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/58G01N33/543C07K14/765C07K14/77C07K14/435C07K16/44C07D401/04
CPCG01N33/94G01N33/577G01N33/581G01N33/54326C07K14/765C07K14/77C07K14/435C07K16/44C07D401/04Y02P20/55
Inventor 周裕军王艳新常缘荣张望周建平
Owner BEIJING DIAGREAT BIOTECH CO LTD