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Immunological detection method of imatinib and/or N-demethylated imatinib

A technology for methyl imatinib and immunological detection, which is applied in the field of immunological detection, can solve the problems of unrecognizable specificity and lack of high sensitivity, and achieve simple operation and strong specificity

Active Publication Date: 2021-06-08
BEIJING DIAGREAT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent document does not specify whether to recognize N-desmethyl imatinib, but it cannot be recognized from the description of specificity
[0007] In summary, there is currently a lack of immunological detection methods with high sensitivity and simultaneous determination of imatinib and N-desmethylimatinib

Method used

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  • Immunological detection method of imatinib and/or N-demethylated imatinib
  • Immunological detection method of imatinib and/or N-demethylated imatinib
  • Immunological detection method of imatinib and/or N-demethylated imatinib

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Embodiment Construction

[0062] The present application will be described in further detail below in conjunction with the examples.

[0063] The preparation example of the hapten of imatinib and N-desmethylimatinib

[0064] The structure of the hapten of imatinib and N-desmethylimatinib is shown in formula 10, and the specific synthetic route of the compound of formula 10 is as follows:

[0065]

[0066] Using carboxybenzaldehyde and trimethyl orthoformate as starting materials, the target product imatinib is obtained through seven steps of aldehyde group protection reaction, condensation reaction, reductive amination reaction, substitution reaction, oxidation reaction, and hydrolysis reaction. and the hapten of N-desmethylimatinib.

[0067] Step (i): The compound shown in formula 1 is reacted with trimethyl orthoformate to obtain the compound shown in formula 2 through aldehyde group protection:

[0068]

[0069] Add p-carboxybenzaldehyde (15g, 0.01mol), 150mL methanol, p-toluenesulfonic acid...

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Abstract

The invention relates to the technical field of immunological detection, and particularly discloses an immunological detection method of imatinib and / or N-demethylated imatinib. The method comprises the following steps: uniformly mixing a blood sample, a magnetic particle working solution containing a conjugate formed by coupling an antibody and magnetic particles, and an enzyme-labeled working solution containing a conjugate formed by coupling a hapten and a marker, sequentially carrying out incubation reaction and cleaning steps, adding an indicator solution for color development, and collecting an optical signal, wherein the structure of the hapten is as shown in a formula 10; the antibody is generated by responding to the antigen, the antigen is a conjugate formed by coupling the hapten and a carrier protein, and the indicator solution contains an indicator for quantitatively detecting the concentration of the marker. According to the present invention, imatinib and N-demethylated imatinib can be simultaneously determined, the specificity is strong, the sensitivity is high, the operation is simple, and the time and the labor are saved.

Description

technical field [0001] The present application relates to the technical field of immunological detection, more specifically, it relates to an immunological detection method of imatinib and / or N-desmethylimatinib. Background technique [0002] Imatinib mesylate (imatinib mesylate) is a small molecule tyrosine kinase inhibitor that targets KIT and platelet-derived growth factor receptor (PDGFRA) to exert anti-tumor activity and is mainly used for the treatment of Philadelphia chromosome (Bcr-Abl) positive chronic myelogenous leukemia (CML) and unresectable or metastatic malignant gastrointestinal stromal tumor (GIST) patients. [0003] Imatinib is mainly metabolized into N-desmethylpiperazine derivatives (N-desmethylimatinib) by CYP3A enzyme system in the human body, and the in vitro efficacy of this metabolite is similar to that of the original drug. Therefore, there is a need for a detection method capable of simultaneously determining imatinib and N-desmethylimatinib. In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/577C07D401/04C07K14/765C07K14/77C07K14/47C07K1/107C07K16/44G01N33/535
CPCG01N33/94G01N33/54326G01N33/577C07D401/04C07K14/765C07K14/77C07K14/4717C07K1/1077C07K16/44G01N33/535C07K2317/33C07K2317/92Y02P20/55
Inventor 许秀丽吴鸣月周建平张望
Owner BEIJING DIAGREAT BIOTECH CO LTD