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Detection reagent and detection kit for detecting imatinib and/or N-demethylated imatinib

A methyl imatinib and detection reagent technology, applied in the field of immunological detection, can solve the problems of lack of high sensitivity and specificity, and achieve the effect of strong specificity and simple operation

Active Publication Date: 2021-06-11
BEIJING DIAGREAT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent document does not specify whether to recognize N-desmethyl imatinib, but it cannot be recognized from the description of specificity
[0007] In summary, there is currently a lack of immunological detection methods with high sensitivity and simultaneous determination of imatinib and N-desmethylimatinib

Method used

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  • Detection reagent and detection kit for detecting imatinib and/or N-demethylated imatinib
  • Detection reagent and detection kit for detecting imatinib and/or N-demethylated imatinib
  • Detection reagent and detection kit for detecting imatinib and/or N-demethylated imatinib

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Embodiment Construction

[0067] The present application will be described in further detail below in conjunction with the examples.

[0068] The preparation example of the hapten of imatinib and N-desmethylimatinib

[0069] The structure of the hapten of imatinib and N-desmethylimatinib is shown in formula 10, and the specific synthetic route of the compound of formula 10 is as follows:

[0070]

[0071] Using carboxybenzaldehyde and trimethyl orthoformate as starting materials, the target product imatinib is obtained through seven steps of aldehyde group protection reaction, condensation reaction, reductive amination reaction, substitution reaction, oxidation reaction, and hydrolysis reaction. and the hapten of N-desmethylimatinib.

[0072] Step (i): The compound shown in formula 1 is reacted with trimethyl orthoformate to obtain the compound shown in formula 2 through aldehyde group protection:

[0073] Add p-carboxybenzaldehyde (15g, 0.01mol), 150mL methanol, p-toluenesulfonic acid monohydra...

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Abstract

The invention belongs to the technical field of immunological detection, and discloses a detection reagent for detecting imatinib and / or N-demethylated imatinib. The detection reagent comprises an enzyme-labeled working solution, a magnetic particle working solution and an indicator solution; the enzyme-labeled working solution comprises a conjugate obtained from a hapten and a marker; the magnetic particle working solution comprises a conjugate obtained by coupling an antibody and magnetic particles; the indicator solution contains an indicator for quantitatively detecting the concentration of the marker; the structure of the hapten is shown as a formula 10 decried in the specifications; and the antibody is generated in response to an antigen, and the antigen is a conjugate formed by coupling the hapten and a carrier protein. According to the detection reagent and detection kit of the present invention, the imatinib and N-demethylated imatinib can be simultaneously determined, and the specificity and the sensitivity of the detection reagent and detection kit are strong.

Description

technical field [0001] The present application relates to the technical field of immunological detection, more specifically, it relates to a detection reagent and a detection kit for detecting imatinib and / or N-desmethylimatinib. Background technique [0002] Imatinib mesylate (imatinib mesylate) is a small molecule tyrosine kinase inhibitor that targets KIT and platelet-derived growth factor receptor (PDGFRA) to exert anti-tumor activity and is mainly used for the treatment of Philadelphia chromosome (Bcr-Abl) positive chronic myelogenous leukemia (CML) and unresectable or metastatic malignant gastrointestinal stromal tumor (GIST) patients. [0003] Imatinib is mainly metabolized into N-desmethylpiperazine derivatives (N-desmethylimatinib) by CYP3A enzyme system in the human body, and the in vitro efficacy of this metabolite is similar to that of the original drug. Therefore, there is a need for a detection method capable of simultaneously determining imatinib and N-desmet...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/58G01N33/543C07K14/765C07K14/77C07K14/435C07K16/44C07D401/04
CPCG01N33/94G01N33/577G01N33/581G01N33/54326C07K14/765C07K14/77C07K14/435C07K16/44C07D401/04Y02P20/55
Inventor 周裕军王艳新常缘荣张望周建平
Owner BEIJING DIAGREAT BIOTECH CO LTD