Method for quantitatively analyzing fluorobenzene series and cyclohexane liquid crystal monomers based on GC-MS/MS technology
A quantitative analysis and liquid crystal monomer technology, applied in the field of chemical detection, can solve problems such as environmental hazards, complex liquid crystal components, systematic qualitative and quantitative detection of fluorobenzene series and cyclohexane liquid crystal monomers, etc., and achieve high precision And stable, with many types of detection and high precision
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Embodiment 1
[0026] A method for quantitatively analyzing fluorobenzene series substances and cyclohexane substances (liquid crystal monomers) based on GC-MS / MS technology, comprising the steps of:
[0027] S1. Pretreatment of fish liver samples: store the collected liver samples in a liquid nitrogen tank at -190±10°C for 30 minutes to quench the metabolic reaction, and then transfer them to a refrigerator at -80±5°C for storage. The liver sample taken out of the test tube was placed in a mortar, added liquid nitrogen to freeze and ground into powder, passed through a 100-mesh sieve, put into a 15mL centrifuge tube, and stored in a -80°C refrigerator for analysis; weighed about 20 mg of the processed sample , add 3.5mL of dichloromethane, shake for 15min, ultrasonically extract for 20min, then centrifuge at 4500r / min for 8min, transfer the supernatant to a clean glass tube, and repeat the extraction process 3 times. The combined supernatants were concentrated and replaced with n-hexane to ...
Embodiment 2
[0031] A method for quantitatively analyzing fluorobenzene series substances and cyclohexane substances (liquid crystal monomers) based on GC-MS / MS technology, comprising the steps of:
[0032] S1. Pretreatment of dust samples: Weigh about 20 mg of dust samples through a 100-mesh sieve, add 3.5 (± 0.5) mL of dichloromethane, shake for 15 (± 2) min, ultrasonically extract for 20 (± 2) min, and then use 4500r / min (±500) for 8 (±2) min, transfer the supernatant to a clean glass tube, and repeat the extraction process 3 (±1) times. The combined supernatants were concentrated and replaced with n-hexane to 0.5 mL under a gentle flow of nitrogen. The concentrated supernatant was purified with columns of 3.0 g florisil (5% water deactivated) and 1 g anhydrous sodium sulfate from bottom to top. The column was activated with 25mL of dichloromethane and 25mL of n-hexane, then the concentrated supernatant was added, rinsed with 20mL of dichloromethane, the eluate was collected, concentr...
Embodiment 3
[0036] A method for quantitatively analyzing fluorobenzene series substances and cyclohexane substances (liquid crystal monomers) based on GC-MS / MS technology, comprising the steps of:
[0037] S1. Pretreatment of samples in fish liver: store the collected liver samples in a liquid nitrogen tank at -190 ± 10°C for 30 minutes to quench the metabolic reaction, and then transfer them to a refrigerator at -80 ± 5°C for storage. The liver sample taken out of the refrigerator was placed in a mortar, added liquid nitrogen to freeze and ground into powder, passed through a 100-mesh sieve, put into a 15mL centrifuge tube, and stored in a -80°C refrigerator for analysis; weighed about 20 mg of the treated liver Add 4.0 mL of dichloromethane to the sample, shake for 15 min, ultrasonically extract for 20 min, then centrifuge at 4800 r / min for 8 min, transfer the supernatant to a clean glass tube, and repeat the extraction process 3 times. The combined supernatants were concentrated and re...
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