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Method for separating and purifying ganoderma lucidum beta-glucan oligosaccharide

A technology for separation and purification of glucooligosaccharides, which is applied in the field of natural medicinal chemistry, can solve problems such as separation and purification difficulties, and achieve the effects of good purity, high yield, and stable product quality

Active Publication Date: 2021-06-18
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the wide distribution of the degree of polymerization of the obtained Ganoderma lucidum β-glucooligosaccharide and the small difference in the structure of oligosaccharides with different degrees of polymerization, it is difficult to separate and purify it.

Method used

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  • Method for separating and purifying ganoderma lucidum beta-glucan oligosaccharide
  • Method for separating and purifying ganoderma lucidum beta-glucan oligosaccharide
  • Method for separating and purifying ganoderma lucidum beta-glucan oligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Gradual alcohol precipitation separation and purification of Ganoderma lucidum β-oligosaccharide

[0028] Weigh 300 mg of Ganoderma lucidum β-oligosaccharide GLPW, add 10 mL of distilled water to prepare a 30 mg / mL solution of Ganoderma lucidum β-oligosaccharide, and stir well until dissolved.

[0029] Gradual alcohol precipitation separation of Ganoderma lucidum β-gluco-oligosaccharide GLPW: slowly add 90 mL of absolute ethanol to the solution until the final concentration of ethanol is 90% (v / v), stir well, let stand at 4°C for 4 hours, and collect by centrifugation The supernatant was washed by adding 50 mL of 90% (v / v) ethanol to the precipitate, and the supernatant was collected by centrifugation. After repeating 3 times, the supernatant was combined and concentrated under reduced pressure to remove the ethanol to obtain the GLPW-A fraction, which was freeze-dried. Add 10 mL of deionized water to the above-mentioned precipitation, fully stir until it is ...

Embodiment 2

[0030] Example 2: Gradual alcohol precipitation separation and purification of Ganoderma lucidum β-oligosaccharide

[0031] Weigh 500 mg of Ganoderma lucidum β-oligosaccharide GLPW, add 10 mL of distilled water to prepare a 50 mg / mL solution of Ganoderma lucidum β-oligosaccharide, and stir well until dissolved.

[0032] Gradual alcohol precipitation separation of Ganoderma lucidum β-gluco-oligosaccharide GLPW: slowly add 90 mL of absolute ethanol to the solution until the final concentration of ethanol is 90% (v / v), stir well, let stand at 4°C for 4 hours, and collect by centrifugation The supernatant was washed by adding 50 mL of 90% (v / v) ethanol to the precipitate, and the supernatant was collected by centrifugation and repeated 3 times. Add 10 mL of deionized water to the above-mentioned precipitation, stir fully until completely dissolved, slowly add 57 mL of absolute ethanol to the solution until the final concentration of ethanol is 85% (v / v), stir well and let stand at...

Embodiment 3

[0033] Example 3: Gradual alcohol precipitation separation and purification of Ganoderma lucidum β-oligosaccharide

[0034] Weigh 400 mg of Ganoderma lucidum β-oligosaccharide GLPW, add 10 mL of distilled water to prepare a 40 mg / mL solution of Ganoderma lucidum β-oligosaccharide, and stir well until dissolved.

[0035] Gradual alcohol precipitation and separation of Ganoderma β-oligosaccharide GLPW: slowly add 90 mL of absolute ethanol to the β-glucose oligosaccharide solution until the final concentration of ethanol is 90% (v / v), stir well and then keep it at 4°C Set aside for 4 h, collect the supernatant by centrifugation, wash the precipitate with 50 mL of 90% (v / v) ethanol, and repeat the centrifugation for 3 times, then combine the supernatant, concentrate under reduced pressure to remove the ethanol to obtain the GLPW-A fraction, and freeze-dry. Add 10 mL of deionized water to the above-mentioned precipitation, stir fully until completely dissolved, slowly add 57 mL of ...

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Abstract

The invention discloses a method for separating and purifying ganoderma lucidum beta-glucan oligosaccharide. The method comprises the following steps: separating a ganoderma lucidum beta-glucan oligosaccharide solution into oligosaccharide fragments with different polymerization degrees by a step-by-step alcohol precipitation method; and analyzing the separated fragments by using high performance gel exclusion chromatography, high performance anion chromatography and mass spectrometry to determine the polymerization degree information of each product. According to the method for separating and purifying the ganoderma lucidum beta-glucan oligosaccharide, the separation and purification effect of the ganoderma lucidum beta-glucan oligosaccharide can be effectively improved, an oligosaccharide fragment with narrow polymerization degree distribution is obtained, and an active fragment of the ganoderma lucidum beta-glucan oligosaccharide is determined in an in-vitro immune experiment.

Description

technical field [0001] The application belongs to the technical field of natural medicinal chemistry, and specifically relates to a method for separating and purifying Ganoderma lucidum β-gluco-oligosaccharide. Background technique [0002] Ganoderma lucidum has been known as "fairy grass" and "sweet grass" since ancient times. In ancient times, Ganoderma lucidum was often believed to prolong life. Ganoderma lucidum is rich in nutrients and has high medicinal value. It has a history of more than 4,000 years of traditional Chinese medicine application in my country. As one of the most abundant components in Ganoderma lucidum, β-glucan of Ganoderma lucidum has a wide range of pharmacological effects, such as immune regulation, anti-cancer and anti-inflammatory. Ganoderma lucidum β-oligosaccharide is a series of fragments with lower molecular weight and better water solubility obtained from the degradation of Ganoderma lucidum β-glucan. The biological activity of β-oligoglucos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02C07H3/06C07H1/08C07H3/04
CPCC08B37/0024C08B37/0003C07H3/06C07H1/08C07H3/04
Inventor 刘艳芳秦秀张劲松颜梦秋唐庆九周帅冯杰王晨光周靖刘利平
Owner SHANGHAI ACAD OF AGRI SCI
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