Method for breaking cell walls of cistanche deserticola to improve cistanche deserticola extraction efficiency

An extraction efficiency and technology of Cistanche deserticola, applied in the field of plant active ingredient extraction, can solve the problems of difficult preservation of finished Cistanche deserticola, low absorption rate of active ingredients, etc., and achieve the effects of good quality, improved extraction rate, and guaranteed purity

Inactive Publication Date: 2021-06-25
HETIAN DCM MEDICINE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above prior art, the present invention provides a method for breaking the cell wall of Cistanche to improve the extraction efficiency of Cistanche, so as to solve the problems of low absorption rate of active ingredients of Cistanche for direct consumption and difficulty in preservation of finished products of Cistanche

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  • Method for breaking cell walls of cistanche deserticola to improve cistanche deserticola extraction efficiency

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Embodiment 1

[0033] A method for breaking the cell wall of Cistanche to improve the extraction efficiency of Cistanche, comprising the following steps:

[0034] S1: Clean Cistanche and dry until the water content is about 12%; then place Cistanche in a vacuum tank, vacuumize and control the temperature in the tank to 55°C, take it out after vacuum treatment for 3 hours and cool to room temperature;

[0035] S2: Alternately spray the mixed enzyme solution and the ethanol solution with a volume concentration of 75% on the surface of Cistanche after S1 treatment, each time the surface of Cistanche is wet and the droplets do not fall; Mix the enzyme solution, and then spray the ethanol solution. The interval between spraying the two solutions is 15 minutes, and each solution is sprayed 3 times; the enzymes in the mixed enzyme solution include cellulase, pectinase, protease and lipase , and the concentration of cellulase in the mixed enzyme liquid is 0.4g / mL, the concentration of pectinase is 0...

Embodiment 2

[0040] A method for breaking the cell wall of Cistanche to improve the extraction efficiency of Cistanche, comprising the following steps:

[0041] S1: Clean Cistanche and dry until the water content is about 10%; then place Cistanche in a vacuum tank, vacuumize and control the temperature in the tank to 50°C, take it out after vacuum treatment for 4 hours and cool to room temperature;

[0042]S2: Alternately spray the mixed enzyme solution and the ethanol solution with a volume concentration of 75% on the surface of Cistanche after S1 treatment, each time the surface of Cistanche is wet and the droplets do not fall; Mix the enzyme solution, then spray the ethanol solution, the interval between spraying the two solutions is 10min, and spray each solution 4 times; the enzymes in the mixed enzyme solution include cellulase, pectinase, protease and lipase , and the concentration of cellulase in the mixed enzyme liquid is 0.1g / mL, the concentration of pectinase is 0.2g / mL, the con...

Embodiment 3

[0047] A method for breaking the cell wall of Cistanche to improve the extraction efficiency of Cistanche, comprising the following steps:

[0048] S1: Clean Cistanche and dry until the water content is about 15%; then place Cistanche in a vacuum tank, vacuumize and control the temperature in the tank to 60°C, take it out after vacuum treatment for 2 hours and cool to room temperature;

[0049] S2: Alternately spray the mixed enzyme solution and the ethanol solution with a volume concentration of 75% on the surface of Cistanche after S1 treatment, each time the surface of Cistanche is wet and the droplets do not fall; Mix the enzyme solution, then spray the ethanol solution, the interval between spraying the two solutions is 20min, and spray each solution twice; the enzymes in the mixed enzyme solution include cellulase, pectinase, protease and lipase , and the concentration of cellulase in the mixed enzyme liquid is 0.5g / mL, the concentration of pectinase is 0.05g / mL, the con...

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Abstract

The invention discloses a method for improving the extraction efficiency of cistanche by breaking cell walls of cistanche, and the method comprises the following steps: placing cistanche in a vacuum tank for vacuum treatment to break walls, and alternately spraying a mixed enzyme solution and an ethanol solution onto the cistanche to digest the cell walls and cell membranes; soaking the cistanche deserticola subjected to enzyme treatment in ethanol for a period of time, grinding into thick liquid, performing ultrasonic oscillation on the thick liquid, and performing filtering, purifying and drying to obtain the cistanche deserticola extract. According to the method, vacuum wall breaking, enzymolysis wall breaking and ethanol solubilization treatment are carried out on the cistanche, so that effective components in the cistanche are extracted more easily, and the extraction rate of the effective components of the cistanche is increased. And subsequent impurity removal treatment is carried out, so the purity of the cistanche extract is ensured, and the cistanche extract with good quality can be obtained.

Description

technical field [0001] The invention belongs to the technical field of extracting active ingredients of plants, and in particular relates to a method for breaking the cell wall of Cistanche deserticola to improve the extraction efficiency of Cistanche deserticola. Background technique [0002] Cistanche deserticola is a parasitic plant that lives on the root of the desert tree Haloxylon. It absorbs nutrients and water from the Haloxylon host. It is known as "desert ginseng". It has extremely high medicinal value and is a traditional Chinese medicinal material. [0003] The extract of Cistanche deserticola has a high medicinal effect on the human body, but every 100 grams of dried Cistanche deserticola contains only about 10 grams of active ingredients such as total glycosides of Cistanche deserticola. The amount is too large. Therefore, people directly eat Herba Cistanche, which cannot bring into play its true medicinal and health-care effects. Moreover, the common product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/64
CPCA61K36/64A61K2236/10A61K2236/333A61K2236/19A61K2236/51A61K2236/55
Inventor 游林贺金华毛艳俞新周佳佳
Owner HETIAN DCM MEDICINE BIOLOGICAL TECH
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