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Test strip for detecting auramine O drugs as well as preparation method and application of test strip

A technology of test strips and auramine, which is applied in the field of test strips for detecting auramine O drugs, can solve the problems of long experiment time, high experiment cost, complicated operation process, etc., and achieve simple and convenient operation, high sensitivity, highly specific effect

Inactive Publication Date: 2021-06-25
广州智汇生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, there is no rapid detection kit for auramine O on the market. If the above-mentioned experimental method is used, the operation process is relatively complicated, the experimental time is long, the experimental cost is high, and it is not suitable for on-site detection.

Method used

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  • Test strip for detecting auramine O drugs as well as preparation method and application of test strip

Examples

Experimental program
Comparison scheme
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preparation example Construction

[0026] The embodiment of the present invention also proposes a preparation method of the above-mentioned test strip, which includes the following steps: pasting filter sample paper, immune colloidal gold paper sheet, immune nitrocellulose membrane, and absorbent paper on the substrate in order to prepare the test paper strip.

[0027] In some embodiments, the preparation of immune colloidal gold disc comprises the following steps:

[0028] The immune colloidal gold solution is prepared; the glass fiber paper is soaked with the pretreatment solution, dried, and then the pretreated glass fiber paper is sprayed with the immune colloidal gold solution, and dried to obtain the immune colloidal gold paper sheet.

[0029] In some embodiments, the preparation of the immune colloidal gold solution comprises the following steps: diluting the anti-auramine O monoclonal antibody labeled with colloidal gold with a gold spray buffer to obtain the OD 540 The immune colloidal gold solution w...

Embodiment 1

[0059] A preparation method for auramine O colloidal gold detection kit, comprising the following steps:

[0060] 1. Preparation of colloidal gold

[0061] The colloidal gold used in the examples of the present invention can be purchased from the market or prepared by the following trisodium citrate reduction method.

[0062] Trisodium citrate reduction method prepares colloidal gold, comprises the following steps (configuring various aqueous solutions all adopts double-distilled or triple-distilled deionized water):

[0063] Prepare HAuCl at a concentration of 0.01% 4 Aqueous solution 100ml, water bath or oil bath to 99 ℃, control temperature constant, then add 1% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 0) aqueous solution 1.80ml, continue to stir and heat about 20min, ultrasonic oscillation, frequency is 25kHz, after 3min, cool to 4.0 ℃ of water baths and keep temperature constant, promptly make the colloidal gold solution of particle diameter about 30nm;

[0064] 2....

Embodiment 2

[0082] A method for rapid detection of auramine O residues in meat products, comprising the following steps:

[0083] 1. Sample preparation: Weigh 5g of the homogenized tissue sample and put it into a 15ml centrifuge tube;

[0084] 2. Standard product preparation: take auramine O veterinary drug powder (98% content, get 0.041g, active ingredient is recorded as 0.04g, be dissolved in 10ml water, mix well), preparation concentration is 5ppb, 0.4ppm, 40ppm, A total of 4 solutions with different concentrations of 4000ppm;

[0085] 3. Add 3ml of acetonitrile and 2ml of trichloroacetic acid aqueous solution into a 15ml centrifuge tube, shake and mix well for 3 minutes, and centrifuge at 4000 rpm for 5 minutes;

[0086]4. Use a 0.5ml disposable straw to take the layered supernatant (do not suck the lower layer) and add all (about 2.5ml) into the supporting 5ml centrifuge tube, and blow dry with air (or nitrogen) at 65°C (centrifuge tube It is normal to have some granular undried ye...

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Abstract

The invention discloses a test strip for detecting auramine O drugs as well as a preparation method and application of the test strip. The test strip comprises a substrate, filter sample paper, an immune colloidal gold paper sheet, an immune nitrocellulose membrane and absorbent paper, and the filter sample paper, the immune colloidal gold paper sheet, the immune nitrocellulose membrane and the absorbent paper are sequentially connected end to end and fixed on the substrate; the immune colloidal gold paper sheet is provided with an anti-auramine O monoclonal antibody marked by colloidal gold, and the immune nitrocellulose membrane is provided with a detection line coated with an auramine O-bovine serum albumin compound and a quality control line coated with goat anti-rabbit IgG. The environment temperature required for using the test strip is 4-35 DEG C, and the test strip is suitable for individuals, factories, food hygiene quality inspection departments, customs and the like to apply auramine O drugs left in medicinal materials, traditional Chinese medicine decoction pieces and food, and is especially suitable for rapid detection of residual auramine O components in meat food; and the test strip also has the advantages of high specificity, high sensitivity, simplicity and convenience in operation and the like.

Description

technical field [0001] The invention belongs to the field of food safety detection, and specifically relates to a test strip for detecting auramine O drugs, a preparation method and an application thereof. Background technique [0002] Auramine O, also known as base light yellow, canary yellow, Austrian yellow, basic Huaihuang, etc., is a chemical pigment and is listed as a human carcinogenic compound by the International Cancer Research Institute (IARC). It is mainly used for the dyeing of acetate fiber and cotton fabrics, and the coloring of paper, leather, paint, etc. Auramine O has a certain toxic effect on the human body, has mild irritation to the skin and mucous membranes, and can cause conjunctivitis, dermatitis, and upper respiratory tract irritation symptoms. Long-term excessive consumption will cause damage to the human kidney and liver and even cause cancer. The Ministry of Health as early as 2008 listed it as a non-edible substance. [0003] Health preservatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/577G01N33/558
CPCG01N33/558G01N33/577G01N33/587
Inventor 李丰毓志超江燕玲文永贤杨韵
Owner 广州智汇生物科技有限公司