Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Protein with cholesterol lowering effect and preparation method thereof

A cholesterol-lowering and effective technology, applied in the field of protein with cholesterol-lowering effect and its preparation, can solve the problems of high cost, time-consuming and labor-intensive, lack of scientificity and credibility, etc.

Inactive Publication Date: 2021-07-06
SOUTH CHINA UNIV OF TECH
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process in this patent is relatively complicated, involves multiple modification methods and belongs to a high-energy input system, which is time-consuming, labor-intensive, and costly
In addition, it is not scientific and credible to judge whether a product has a cholesterol-lowering effect only by measuring the solubility inhibition rate of cholesterol micelles

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Protein with cholesterol lowering effect and preparation method thereof
  • Protein with cholesterol lowering effect and preparation method thereof
  • Protein with cholesterol lowering effect and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Accurately weigh 5g of soybean protein isolate powder, disperse it in 100g of distilled water, adjust the pH to 7.0, stir continuously at room temperature (25°C) for 2h to dissolve completely, and then hydrate at 4°C for 12h , to obtain a concentration of 5wt% soybean protein isolate solution.

[0038] 2) Take out the soybean protein isolate solution in 1) and return to room temperature, centrifuge to remove insoluble matter to obtain a protein solution; heat the protein solution at a constant temperature of 60°C, 95°C and 120°C for 20 minutes, and then immediately ice-bath to make The temperature is returned to room temperature (25° C.), and the cooling rate is 3° C. / min to obtain a heat-pretreated soybean protein isolate dispersion.

[0039] 3) Centrifuge the heat-pretreated soybean protein dispersion obtained in 2) (5000 g, 10 min), and take the supernatant to obtain a clear solution.

[0040] 4) The clear solution obtained in 3) is freeze-dried to obtain a prote...

Embodiment 2

[0047] 1) Accurately weigh 5 soybean 11s protein powders, disperse them in 100g of distilled water, adjust the pH to 7.0, stir continuously at room temperature (25°C) for 2h to dissolve completely, and then hydrate at 4°C for 12h , to obtain a soybean 11s protein solution with a concentration of 5wt%.

[0048] 2) Take out the soybean 11s protein solution in 1), wait until it returns to room temperature, and centrifuge to remove insoluble matter to obtain a protein solution; heat the protein solution at a constant temperature of 60°C, 95°C and 120°C for 20 minutes, and then immediately ice-bath to make The temperature of the sample was brought to room temperature (25° C.), and the cooling rate was 3° C. / min to obtain a preheated soybean 11s protein dispersion.

[0049] 3) centrifuge (5000g, 10min) the heat-pretreated soybean 11s protein dispersion obtained in 2), and take the supernatant to obtain a clear solution.

[0050] 4) The clear solution obtained in 3) is freeze-dried ...

Embodiment 3

[0054] 1) Accurately weigh 5g of soybean 7s protein powder, disperse it in 100g of distilled water, adjust the pH to 7.0, stir continuously at room temperature (25°C) for 2h to dissolve completely, and then hydrate at 4°C for 12h to obtain a stock solution of soybean 7s protein with a concentration of 5wt%.

[0055] 2) Take out the soybean 7s protein solution in 1), wait until it returns to room temperature, and centrifuge to remove insoluble matter to obtain a protein solution; heat the protein solution at 60°C, 95°C and 120°C for 20 minutes at a constant temperature, and then immediately ice-bath to make After it is cooled to room temperature, the heat-pretreated soybean 7s protein dispersion is obtained.

[0056] 3) Centrifuge (5000g, 10min) the heat-pretreated soybean 7s protein dispersion obtained in 2), and take the supernatant to obtain a clear solution.

[0057] 4) The clear solution obtained in 3) is freeze-dried to obtain a protein product (the protein having a chol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of functional food preparation, and discloses a protein with a cholesterol lowering effect and a preparation method thereof. The preparation method comprises the following steps: adding water into soybean protein for dispersing, and hydrating to obtain a protein solution; preheating the protein solution to obtain a preheated protein solution; carrying out ice bath on the preheated protein solution to recover the temperature to room temperature, and then centrifuging to remove insoluble substances to obtain clear liquid; and freeze-drying the clear liquid in vacuum freeze-drying equipment to obtain a finished product. A complete in-vitro digestion model verifies that the soybean protein product prepared by the invention has better cholesterol binding capacity and cholate binding capacity compared with natural soybean protein, can effectively reduce the solubility of bile micelles, and is the product with the cholesterol lowering effect. According to the method disclosed by the invention, the product with an excellent cholesterol lowering effect can be obtained only through preheating treatment on the premise that high energy input is not needed, and the method is a green and economic preparation process with high biological retention activity.

Description

technical field [0001] The invention belongs to the technical field of soybean protein deep processing, and in particular relates to a protein with cholesterol-lowering effect and a preparation method thereof. Background technique [0002] Hyperlipidemia (HLP) is a systemic metabolic chronic disease caused by abnormal metabolism of fat substances in blood such as cholesterol, triglycerides and lipoproteins. It is an important factor of common metabolic diseases and seriously endangers human health. About 2.5 million to 3 million people die from such diseases in my country every year. Studies have shown that the risk of HLP will increase by about 30% for every liter of total cholesterol content in serum increased by 0.6mM. Therefore, maintaining cholesterol levels within a healthy range can greatly reduce the chances of developing HLP. At present, there are many chemical drugs for the treatment of HLP on the market. Although they have clear mechanisms and targets, they hav...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23L33/185
CPCA23L33/185A23V2002/00A23V2200/3262A23V2250/5488
Inventor 唐传核朱雯雯
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com