Liquorice detection method for veterinary motherwort biochemical mixture
A technology for biochemical and detection methods of mother-of-pearl is applied in the field of licorice detection of veterinary mother-of-pearl biochemical compounds, which can solve the problems that the processing method is not precise enough, cannot be accurately defined, etc., and achieves judgment, easy judgment, and specificity that are convenient for color development. strong effect
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Embodiment 1
[0023] In Example 1, a licorice detection method of a beast-based nominal compound, including the following steps:
[0024] S1, the preparation of the test solution: take 30ml of the test material, adsorb the resin column by D101 macropore, 150 ml of water, then eluate 40 mL, collect eluting, steam, residue plus methanol 3ml dissolved, take the supernatant as a test solution;
[0025] Preparation of S2, licorice antiques: Take 0.8 g of the licorice antistories, 40 ml of water, heat reflux for 45min, filtered, filtrate to 5 mL, add 90% ethanol, allowed by 85%, standing for 32h, Filtered, the filtrate was evaporated, the residue was dissolved 8 ml, and the resin column was adsorbed by D101 macropore, and then washed with water 150 mL, and then eluted 40 mL was eluted, and the eluate, steamed, and residual residue added 3ml of methanol. Take the supernatant as a solution of licorice drugs;
[0026] S3, thin layer chromatographic identification: 6 μl of the test solution, 12 μl of the...
Embodiment 2
[0032] In Example 2, a licorice detection method of a beast-based nominal biochemical agent, including the following steps:
[0033] S1, the preparation of the test solution: 10ml of the test material, adsorb resin column by D101 macropore, 50 mL of water, 20 ml of eluate, mix eluent, steam, residue plus methanol 1 ml dissolved, take the supernatant as the test solution;
[0034] Preparation of S2, licorice antiques: Take 0.3 g of the licorice drugs, add water 20L, heat reflux for 15 min, filtrate, add 90% ethanol, allowed by 85%, and stand for 18h, Filtered, the filtrate was evaporated, the residue was dissolved, and the resin column was adsorbed by D101, washed 50 ml of water, and then eluted 20 mL, collect eluent, steamed dry, residue plus methanol 1 ml dissolved, Take the supernatant as a solution of licorice drugs;
[0035] S3, thin layer chromatography: 2 μl of the solution solution, 6 μl of 12 μl of the test solution, points on the same thin layer chromatography, with a vol...
Embodiment 3
[0041] In Example 3, the method of detecting the mother-induced compound, including the following steps:
[0042]S1, the preparation of the test solution: 20 ml of the test product, adsorb the resin column by D101 macropole, washed 100 ml of water, then eluent 30 mL, collect eluting, steam, residue plus methanol 2 ml dissolved, take the supernatant as a test solution;
[0043] Preparation of S2, licorice antiques: 0.5 g of the licorice antiques, 30 ml of water, heat reflux for 30 min, filtrate, the filtrate was concentrated to 4 ml, add 90% ethanol, allowed by 85%, standing 24h, Filtered, the filtrate was evaporated, the residue was dissolved, and the resin column was dissolved. After the D101 macroporous, the resin column was absorbed, and after 100 ml of water, it was eluted with the eluate 30 mL, collecting eluent, steam, residue plus methanol 2 ml dissolved, Take the supernatant as a solution of licorice drugs;
[0044] S3, thin layer chromatography: 4 μl of the test solution,...
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