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Method for separating and purifying geniposidic acid by using novel composite imprinted microspheres

A technology for the separation and purification of geniposidic acid, which is applied in the field of solid phase extraction separation and purification, and the preparation of new imprinted microspheres. It can solve the problems of slow molecular recognition and mass transfer process, application limitations, etc., and achieve high product recovery rate, High-efficiency separation ability and the effect of reducing production cost

Pending Publication Date: 2021-07-13
李辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Molecularly imprinted polymers obtained by conventional imprinting technology are greatly limited in application due to the deep burial of binding sites, which leads to slow molecular recognition and mass transfer processes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Preparation of cadmium tellurium doped quantum dots (CdTe QDs)

[0021] Weigh 28.1 mg of NaBH 4 and 6.0 mg tellurium powder were placed in a centrifuge tube, purged with nitrogen for 5 minutes, added 10 mL of distilled water saturated with nitrogen, and sonicated for 1 hour under anaerobic conditions to obtain a colorless or light pink NaHTe solution (solution A). Weigh 53.6 mg of CdCl 2 • 2H 2 O, added to 40 mL of distilled water, then added 32 μL of 98% 3-mercaptopropionic acid (MPA), adjusted the pH of the mixed solution to 12.0 with 1M NaOH, and passed nitrogen gas for 10 minutes to obtain liquid B. Quickly pour the newly prepared solution A into solution B, and react in a water bath at 100°C for 50 minutes to obtain a solution containing cadmium and tellurium doped quantum dots, and place it in a refrigerator at 4°C for later use.

[0022] (2) Preparation of metal-organic framework ZIF-67

[0023] 12.0 mmol Co(NO 3 ) 2 •6H 2 O was dissolved in 30.0 mL me...

Embodiment 2

[0028] (1) Preparation of cadmium tellurium doped quantum dots (CdTe QDs)

[0029] Weigh 35.0 mg of NaBH 4 and 7.7 mg of tellurium powder were placed in a centrifuge tube, purged with nitrogen for 5 minutes, added 12-15 mL of distilled water saturated with nitrogen, and sonicated for 1 hour under anaerobic conditions to obtain a colorless or light pink NaHTe solution (liquid A) . Weigh 56.5 mg of CdCl 2 • 2H 2 O, added to 40 mL of distilled water, then added 35-45 μL of 98% 3-mercaptopropionic acid (MPA), adjusted the pH of the mixed solution to 12.0 with 1 M NaOH, and passed nitrogen gas for 10 minutes to obtain liquid B. Quickly pour the newly prepared solution A into solution B, and react in a water bath at 100°C for 50 minutes to obtain a solution containing cadmium and tellurium doped quantum dots, and place it in a refrigerator at 4°C for later use.

[0030] (2) Preparation of metal-organic framework ZIF-67

[0031] 14.5 mmol Co(NO 3 ) 2 •6H 2 O was dissolved in ...

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Abstract

The invention discloses a preparation method of novel geniposidic acid composite imprinted microspheres and an application of the novel geniposidic acid composite imprinted microspheres to solid-phase extraction separation of geniposidic acid in eucommia ulmoides male flower extracts. The preparation method comprises the steps: (1) preparing cadmium tellurium doped quantum dots; (2) preparing a metal organic framework ZIF-67; (3) preparing geniposidic acid composite imprinted particles; and (4) carrying out solid-phase extraction of the geniposidic acid composite imprinted material. And the geniposidic acid with the purity of 38.5-48.2% is separated from an eucommia ulmoides male flower extracting solution. The cadmium-tellurium-doped quantum dots and the metal organic framework are used as common carriers, geniposidic acid is used as a template, and the composite imprinted material is prepared; the material has efficient separation capacity on geniposidic acid in the eucommia ulmoides male flower water extract, a product with high purity can be obtained through single extraction, and the material can be used for classification and purification of compounds of the type in plants; and the imprinted microspheres can be repeatedly used after being recycled, so that the production cost is reduced.

Description

technical field [0001] The invention belongs to the field of new materials, and in particular relates to a method for preparing novel imprinted microspheres with high selective adsorption capacity for geniposide and solid-phase extraction separation and purification. Background technique [0002] Eucommia ulmoides is a traditional Chinese medicinal material. "Shen Nong's Materia Medica" lists eucommia as the top grade. Eucommia has many functions such as invigorating the middle and nourishing Qi, strengthening bones and muscles, strengthening the will, preventing miscarriage, preventing arteriosclerosis, improving energy, anti-fatigue, compensating for the lack of exercise of mental workers, enhancing immune function, removing garbage in the body, and preventing and fighting cancer. Eucommia geniposide is a biologically active substance extracted from Eucommia male flowers, which has anti-cancer and anti-cancer effects, and can be used to prevent tumors caused by X-rays. Ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/22B01J20/28B01J20/30B01D15/08
CPCB01J20/268B01J20/226B01J20/28011B01J20/28021B01D15/08
Inventor 李辉许国强罗晓玥毛琳田海希
Owner 李辉