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Application of magnetic functionalized magnetic nanomaterial and mixed microorganism identification method

A technology of mixing microorganisms and magnetic nanometers, applied in the field of microorganisms, can solve the problems of long-time cultivation, affecting doctors' diagnosis and treatment and patients' recovery time, etc., and achieves the effect of simple operation, problem-solving and auxiliary medical treatment.

Pending Publication Date: 2021-07-23
CHUAN-MING (NINGBO) CHEM SCITECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing methods for detecting and identifying mixed bacteria in biological samples, especially in urine, are mainly culture, but the culture takes a long time, which directly affects the doctor's diagnosis and treatment and the patient's recovery time
Although matrix-assisted laser desorption / ionization time-of-flight mass spectrometry is used in the identification of various microorganisms, it is currently only used to identify a single bacterium

Method used

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  • Application of magnetic functionalized magnetic nanomaterial and mixed microorganism identification method
  • Application of magnetic functionalized magnetic nanomaterial and mixed microorganism identification method
  • Application of magnetic functionalized magnetic nanomaterial and mixed microorganism identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Enrichment and identification of Escherichia coli and Staphylococcus aureus in different volume ratios in aqueous solution and urine

[0026] Bacterial samples: Escherichia coli (Ec) ATCC 25922, Staphylococcus aureus (Sa) ATCC 25923.

[0027] Specific steps:

[0028] 1. Inoculate the strains frozen at -80°C on Tryptone Soy Agar (TSA) and culture them in a 37°C incubator for 12-14 hours.

[0029] 2. Dissolve the part of the cultured colony in a centrifuge tube filled with sterile water, and the OD of the bacterial solution 600 The value is adjusted to 0.7-0.8. Take a certain volume and put it in another centrifuge tube and centrifuge, then add an equal volume of urine to prepare a specific OD 600 value of monobacterial urine.

[0030] 3. According to the volume ratio of 1:1 and 2:1, take the single bacteria solution and mix them in pairs to obtain the mixed bacteria solution in water and urine with a total volume of 200 μL and 150 μL respectively.

[0031]...

Embodiment 2

[0039] Example 2 Enrichment and Identification of Escherichia coli and Klebsiella pneumoniae Dimixed Bacteria under Different Volume Ratio in Aqueous Solution and Urine

[0040] Bacterial samples: Escherichia coli (Ec) ATCC 25922, Klebsiella pneumoniae (KP) CICC 21519

[0041] Specific steps:

[0042] 1. Inoculate the strains frozen at -80°C on Tryptone Soy Agar (TSA) and culture them in a 37°C incubator for 12-14 hours.

[0043] 2. Dissolve the part of the cultured colony in a centrifuge tube filled with sterile water, and the OD of the bacterial solution 600 The value is adjusted to 0.7-0.8. Take a certain volume and put it in another centrifuge tube and centrifuge, then add an equal volume of urine to prepare a specific OD 600 value of monobacterial urine.

[0044]3. According to the volume ratio of 1:1 and 2:1, take the single bacteria solution and mix them in pairs to obtain the mixed bacteria solution in water and urine with a total volume of 200 μL and 150 μL respec...

Embodiment 3

[0053] Example 3 Enrichment and identification of Escherichia coli and Pseudomonas aeruginosa mixed bacteria in different volume ratios in aqueous solution and urine

[0054] Bacterial samples: Escherichia coli (Ec) ATCC 25922, Pseudomonas aeruginosa (PA) CICC 21630 Specific steps:

[0055] 1. Inoculate the strains frozen at -80°C on Tryptone Soy Agar (TSA) and culture them in a 37°C incubator for 12-14 hours.

[0056] 2. Dissolve the part of the cultured colony in a centrifuge tube filled with sterile water, and the OD of the bacterial solution 600 The value is adjusted to 0.7-0.8. Take a certain volume and put it in another centrifuge tube and centrifuge, then add an equal volume of urine to prepare a specific OD 600 value of monobacterial urine.

[0057] 3. According to the volume ratio of 1:1 and 2:1, take the single bacteria solution and mix them in pairs to obtain the mixed bacteria solution in water and urine with a total volume of 200 μL and 150 μL respectively.

...

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Abstract

The invention relates to an application of a magnetic functionalized magnetic nanomaterial and a mixed microorganism identification method. A capture material is formed by simultaneously connecting mannose binding lectin and immune globulin G to the surface of a magnetic bead; and the mannose binding lectin is recombinant protein, the immune globulin G is natural protein, and the magnetic functionalized magnetic nanomaterial is used for capturing or identifying mixed microorganisms in a sample. By adopting the application and the method provided by the invention, the specific variety of mixed bacterial infection in the sample can be quickly obtained without long-time culture.

Description

technical field [0001] The invention relates to the field of microorganisms, and in particular to the use of a magnetic functionalized magnetic nanometer material and a method for identifying mixed microorganisms. Background technique [0002] At present, the types of bacteria known to humans are limited. Bacteria are a part of microorganisms, and the death caused by public health incidents caused by bacteria is tantamount to disasters. For bacteria, people talk about it. At present, a lot of financial and material resources have been invested in the field of medicine and health to prevent patients from being attacked by bacteria in the surrounding environment, especially drug-resistant bacteria, and then cause secondary infections. How to effectively and quickly identify bacteria is a technical difficulty. For urinary system infection caused by dimixed bacteria, how to directly identify dimixed bacteria in urine has important clinical guidance and practical significance. ...

Claims

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Application Information

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IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 余绍宁施海梅程文敏
Owner CHUAN-MING (NINGBO) CHEM SCITECH CO LTD
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